松质骨孔隙率的二维光电检测

采用二维光电检测方法研究松质骨孔隙率沿不同方向的分布规律。新鲜猪胫骨后肢松质骨制备成36张切片并均分成两组：一组为沿胫骨轴向的横切切片，另一组为垂真于胫骨轴向的纵切切片。经光镜观察和图像分析，计算出每张松质骨切片表面的总面积、骨基质的面积、孔隙的面积及该松质骨切片的孔隙率。测试结果表明：松质骨孔隙率在轴向沿靠近密质骨的方向逐渐减小，在垂直于胫骨轴向方向基本不变；松质骨可看作是横观各向同性材料；如果切片数据足够多，采用二维光电检测方法，能较为准确地测试出松质骨孔隙率沿不同方向的分布规律。

Two-dimension Opto-electronic Test Method to Determine the Cancellous Bone Porosity

A two dimension opto electronic test method has been developed to determine the distribution characteristics of cancellous bone porosity. The fresh pig tibiae concellous bone in behind leg was sectioned. A total of 36 cancellous bone sections were divided two groups. The first group consisted of 18 transversal sections along the axial direction, and the second group consisted of 18 axial sections along the transversal direction of the tibiae. Their images were examined microscopically and processed quantitatively. The total area, solid matrix area and pore area of each specimen and its porosity were determined, too. The cancellous bone porosity is gradually decreased along the axial direction of tibiae and approaching to the compact bone, but almost constant along the transversal direction. Cancellous bone is modeled as a transversely isotropic, liquid saturated porous solid. The two dimension opto electronic test method can be used to relatively accurately determine the distribution characteristics of the cancellous bone porosity from different directions if the cancellous bone sections are enough.