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回流法提取菠萝皮中多酚及其含量的测定
引用本文:宋力,薛灵芬,李森,谢灿,张克立.回流法提取菠萝皮中多酚及其含量的测定[J].信阳师范学院学报(自然科学版),2016(4):592-595.
作者姓名:宋力  薛灵芬  李森  谢灿  张克立
作者单位:信阳师范学院化学化工学院;武汉大学化学与分子科学学院
基金项目:国家自然科学基金项目(61405170);2015年国家级大学生创新创业训练计划项目(201510477013)
摘    要:采用乙醇回流法从菠萝皮中提取多酚类物质,利用酒石酸亚铁分光光度法表征提取物中多酚的含量,通过正交试验对提取条件进行优化,结果表明:菠萝皮中多酚的最佳提取条件为:乙醇浓度为70%,提取温度为30℃,料液比为1∶8(g∶mL),提取时间为45min,菠萝皮中多酚类化合物的提取量为12.65mg/g.

关 键 词:菠萝皮  多酚  抗氧化性  酒石酸亚铁法  正交试验法

Reflux Method to Extract Polyphenols in Pineapple Peel and Polyphenols Content Determination
SONG Li;XUE Lingfen;LI Sen;XIE Can;ZHANG Keli.Reflux Method to Extract Polyphenols in Pineapple Peel and Polyphenols Content Determination[J].Journal of Xinyang Teachers College(Natural Science Edition),2016(4):592-595.
Authors:SONG Li;XUE Lingfen;LI Sen;XIE Can;ZHANG Keli
Institution:SONG Li;XUE Lingfen;LI Sen;XIE Can;ZHANG Keli;College of Chemistry and Chemical Engineering,Xinyang Normal University;College of Chemistry and Molecular Sciences,Wuhan University;
Abstract:Polyphenols was extracted from pineapple peels by ethanol reflux method.The antioxidant level was determined by the color reaction of ferrous tartrate with the polyphenol extract.The best extraction conditions were obtained by orthogonal experiments.The results showed that the optimum extraction conditions were obtained with ethanol concentration of 70%,extraction temperature of 50 ℃,the ratio of material to liquid of 1∶8(g∶mL)and extraction time of 45 min.The amount of Polyphenols extracted from Pineapple Peel was12.65mg/g.
Keywords:pineapple peels  polyphenols  antioxidant  ferrous tartrate  orthogonal experiment
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