| 强阳离子交换整体柱用于蛋白的分离 |
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| 引用本文： | 刘海燕,白立改,杨更亮,杨冠群,封晓娟.强阳离子交换整体柱用于蛋白的分离[J].河北大学学报(自然科学版),2009,29(5). |
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| 作者姓名： | 刘海燕 白立改 杨更亮 杨冠群 封晓娟 |
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| 作者单位： | 1. 河北大学药学院,河北保定,071002
2. 河北大学药学院,河北保定,071002;中国科学院化学研究所分子科学中心,北京,100080 |
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| 基金项目： | 国家自然科学基金资助项目,河北省科技攻关项目,河北省教育厅项目,中国科学院"百人计划"项目 |
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| 摘 要： | 以乙烯基树脂为单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,十二醇为制孔剂,经原位聚合法制备了聚(乙烯基酯树脂-乙二醇二甲基丙烯酸酯)整体柱,然后在该柱上接入γ-缩水甘油基氧丙基三甲氧基硅烷,再用0.1 mol/L HCl和100 g/L NaHSO3处理后制成阳离子交换整体柱.考察了α-淀粉酶和溶菌酶在该柱上的分离,并对蛋清中的蛋白进行了分离.结果表明,等电点不同的蛋白在合适的pH条件下都能得到较好地分离.
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| 关 键 词： | 强阳离子交换 整体柱 蛋白 分离 |
Separation of Proteins Using Strong Cation-exchange Monolith |
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| LIU Hai-yan,BAI Li-gai,YANG Geng-liang,YANG Guan-qun,FENG Xiao-juan.Separation of Proteins Using Strong Cation-exchange Monolith[J].Journal of Hebei University (Natural Science Edition),2009,29(5). |
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| Authors: | LIU Hai-yan BAI Li-gai YANG Geng-liang YANG Guan-qun FENG Xiao-juan |
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| Institution: | 1.College of Pharmacy;Hebei University;Baoding 071002;China;2.Institute of Chemistry;Chinese Academy of Science;Beijing 100080;China |
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| Abstract: | Using vinyl ester resin as monomer,ethylene glycol dimethacrylate(EDMA) as the crosslinker,dodecanol as porogenic solvents and 2,2′-azobisisobutyronitrile(AIBN) as initiators,a poly(vinyl ester resin-co-ethylene dimethacrylate) monolithic column was prepared by "in situ" process.The monolith was further derived by 3-glycidoxypropyltrimethoxysilane and then modified using 0.1 mol/L HCl and 100 g/L NaHSO3 to prepare strong cation-exchange monolithic column.The separation of α-amylase and lysozyme on the monolithic column was investigated.Moreover,proteins in chicken egg white were also separated.The results showed that proteins with different isoelectric point could be separated in appropriate conditions. |
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| Keywords: | strong cation-exchange monolithic column protein separation |
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