| VC二步发酵伴生菌苏云金芽孢杆菌的选育 |
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| 引用本文： | 郭礼强,郭立格,卢育新,张利平.VC二步发酵伴生菌苏云金芽孢杆菌的选育[J].河北大学学报(自然科学版),2006,26(1):108-111. |
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| 作者姓名： | 郭礼强 郭立格 卢育新 张利平 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
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| 摘 要： | 苏云金芽孢杆菌经紫外线(UV)、亚硝基胍(NTG)及两者的复合诱变,获得1株高效伴生菌株UN-366.在pH 6.5~6.8的发酵培养基中与氧化葡萄糖酸杆菌混合发酵,UN-366的平均糖酸转化率提高了6.32%,古龙酸的质量浓度达到了83.7 g/L,经连续5代转接发酵实验,性状稳定.
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| 关 键 词： | VC二步发酵 选育 苏云金芽孢杆菌 复合诱变 |
| 文章编号： | 1000-1565(2006)01-0108-04 |
| 修稿时间： | 2005年8月25日 |
Breeding of Associated Bacteria Bacillus thuringignsis in "Two-step Fermentation" of Vc |
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| GUO Li-qiang,GUO Li-ge,LU Yu-xin,ZHANG Li-ping.Breeding of Associated Bacteria Bacillus thuringignsis in "Two-step Fermentation" of Vc[J].Journal of Hebei University (Natural Science Edition),2006,26(1):108-111. |
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| Authors: | GUO Li-qiang GUO Li-ge LU Yu-xin ZHANG Li-ping |
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| Abstract: | One mutant strain UN-366 with high efficiency of association ability was screened from Bacillus thuringignsis by series mutagenesis methods which were ultraviolet light,NTG and complex of them.The converting rate of L-sorbose to KGA was increased by 6.32% when it was mixed fermentation with Gluconobacter oxydans in medium of pH 6.5～6.8,and the yielding of KGA reached 83.7 g/L.The features of UN-366 were shown stable after it was continuously transferred and fermented for 5 generations. |
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| Keywords: | two-step fermentation of V screening Bacillus thuringignsis comlex mutagenesis |
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