2013年春季北京地区啮齿类实验动物健康状况的病理学调查和分析

目的2013年5月本实验室对北京市14家单位的啮齿类实验动物的健康状况进行病理学调查。方法采集心、肝、脾、肺、肾、大肠和小肠后经10％福尔马林固定，通过石蜡切片H．E．染色、冰冻切片油红0染色、PAS染色于显微镜下观察。结果部分动物出现不同程度的肝细胞肿胀、轻度肺脏病变、肾脏蛋白样渗出物和管型。结论本次抽检说明绝大多数啮齿类实验动物为健康状态，上述病变可能与饲养环境、饲料营养成分以及运输、处死时的应激有关，提高饲养管理水平可加以改善。

Analysis of the Health Status of Beijing Laboratory Rodents in Spring of 2013 by Pathological Evaluation

Objective In May, 2013, our lab has taken samples from 14 units to investigate the health status of Beijing laboratory rodents. Method The heart, liver, spleen, lung, kidney, large intestine and small intestine of animals were collected, and were put into 10% paraformaldehyde solution for fixation, and then two different sections were prepared for optical microscopy observation, including all paraffin specimens stained with H.E. and the frozen sections stained with Oil Red O and PAS. Result Liver cell swelling, slight lung lesion and protein cast of kidney were main lesions which might affect the health of laboratory rodents. Conclusion The results showed that the vast majority of laboratory rodents were pathologically diagnosed as healthy animals. The result might he related to breeding and feeding environment and transport stress, and the improvement of management level can ameliorate the health status of laboratory rodents.