| 萍蓬草的组织培养及快速繁殖的研究 |
| |
| 引用本文： | 李肖依,赵娜,王宇,邹翠霞,姜长阳.萍蓬草的组织培养及快速繁殖的研究[J].科技信息,2008(29):45-46. |
| |
| 作者姓名： | 李肖依 赵娜 王宇 邹翠霞 姜长阳 |
| |
| 作者单位： | 辽宁师范大学生命科学学院 |
| |
| 摘 要： | 以萍蓬草的嫩根茎为外植体,在附加不同浓度的BA、IAA、NAA和2,4-D的MS、1/2MS或1/3MS培养基上进行了愈伤组织的诱导、愈伤组织的分化、不定芽的生根、试管苗的移栽和移植研究,成功的建立起萍蓬草嫩根茎无性系,达到了快速繁殖的目的。结果表明：MS＋BA0.4-0.8mg·L^-1＋2,4-D1.2mg·L^-1是诱导嫩根茎形成具有分化能力愈伤组织的理想培养基;1/2MS＋AgN031.2mg·L^-1＋BA0.3mg·L^-1＋NAA0.1mg·L^-1是诱导颗粒状愈伤组织和不定芽分化的理想培养基;1/3MS＋IAA0.5mg·L^-1的液体培养基是试管苗生根培养和生根继代培养的理想培养基;试管苗移栽平均成活率为99.4%。
|
| 关 键 词： | 萍蓬草 愈伤组织 无性系 |
The study of Tissue culture and rapid propagation of Nuphar pumium(Timm.) DC. |
| |
| LI Xiao-Yi ZHAO Na WANG Yu ZOU Cui-Xia JIANG Chang-Yang.The study of Tissue culture and rapid propagation of Nuphar pumium(Timm.) DC.[J].Science,2008(29):45-46. |
| |
| Authors: | LI Xiao-Yi ZHAO Na WANG Yu ZOU Cui-Xia JIANG Chang-Yang |
| |
| Institution: | (College of life Science, Liaoning Normal University Dalian 116029 china) |
| |
| Abstract: | The tender stem of Nuphar pumium （Timm.） DC. was cultured on medium MS with BA,IAA,NAA and 2, 4-D at different levels. The results showed that MS＋BA0.4-0.8 mg.L^-1＋2,4-D1.2mg.L^-1 was the best medium for inducing callus with stem as explant ; 1/2MS＋AgNO31.2mg.L^-1＋BA0.3mg.L^-1＋NAA0.1mg.L^-1 was the most suitable medium for shoot differentiation;1/3MS＋AgNO31.2mg.L^-1＋BA0.3mg.L^-1＋NAA0.1mg. L^-1 was the optimum medium for rooting. The survival percent of transplantation could reach 99.4%. |
| |
| Keywords: | Nuphar pumium(Timm )DC callus clone |
| 本文献已被 CNKI 维普 等数据库收录！ |
