| 红霉素发酵过程前期参数相关分析及调控 |
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| 引用本文： | 庄英萍,储炬,张嗣良,赵见喜,杨生武.红霉素发酵过程前期参数相关分析及调控[J].华东理工大学学报(自然科学版),2004,30(6):636-639. |
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| 作者姓名： | 庄英萍 储炬 张嗣良 赵见喜 杨生武 |
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| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心(上海),上海,200237
2. 湖南岳阳中湘康神药业有限公司,岳阳,414000 |
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| 基金项目： | 上海市科委重点科技攻关项目(034319220) |
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| 摘 要： | 对红霉素发酵过程前期调控策略进行了研究，从宏观代谢流的变化着手，对表征细胞代谢特征的参数进行了相关分析，对发酵前期的摄氧率(OUR)、二氧化碳释放率(CER)及呼吸商(RQ)相关变化及红霉素发酵前期CER与pH的变化进行了分析，确定了合适的调控方式，在工业规模发酵罐上通过控制发酵过程前期补糖速率等措施，使发酵平均水平从原来的4500u／mL提高到了6000u／mL。
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| 关 键 词： | 红霉素 发酵 摄氧率 呼吸商 参数相关 |
| 文章编号： | 1006-3080(2004)06-0636-04 |
| 修稿时间： | 2003年12月21 |
Parameter Correlation Analysis and Optimization of Process in the Early Period of Erythromycin Fermentation |
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| ZH UANG Ying-ping ,CH U Ju,ZH ANG Si-liang,ZH AO Jian-xi,YANG Sheng-wu.Parameter Correlation Analysis and Optimization of Process in the Early Period of Erythromycin Fermentation[J].Journal of East China University of Science and Technology,2004,30(6):636-639. |
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| Authors: | ZH UANG Ying-ping CH U Ju ZH ANG Si-liang ZH AO Jian-xi YANG Sheng-wu |
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| Institution: | ZH UANG Ying-ping1 *,CH U Ju1,ZH ANG Si-liang1,ZH AO Jian-xi2,YANG Sheng-wu2 |
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| Abstract: | The metabolic feature of the early period fermentation process of erythromycin were studied. Monitoring of some online parameters, such as oxygew uptake rate (OUR), carbon dioxide exit rate (CER), respiratory quotient (RQ), which are characteristics of macro-metabolic flux, is the basis of the optimization of bioprocess. The stringent glucose feeding strategies in the early phase was adopted and the productivity of erythromycin was raised to 6 000 u/mL from 4 500 u/mL. |
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| Keywords: | erythromycin fermentation oxygen uptake rate respiratory quotient parameter correlation analysis |
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