| 低强度脉冲超声对MC3T3-E1细胞增殖、分化和矿化的影响 |
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| 引用本文： | 王然,马俊,詹欣,辛敏,王婕,王晓文,唐劲天,岳秉飞.低强度脉冲超声对MC3T3-E1细胞增殖、分化和矿化的影响[J].科技导报(北京),2013,31(32):64-67. |
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| 作者姓名： | 王然 马俊 詹欣 辛敏 王婕 王晓文 唐劲天 岳秉飞 |
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| 作者单位： | 1. 北京中医药大学生物制药系, 北京 100102;2. 清华大学工程物理系, 北京 100084;3. 中国食品药品检定研究院实验动物资源研究所, 北京 100050 |
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| 摘 要： | 为了研究低强度脉冲超声促进MC3T3-E1细胞增殖、分化和矿化的有效性,初步筛选频率和强度参数,分别对细胞施加不同参数(频率、强度)的超声,采取CCK-8法检测细胞的增殖情况,碱性磷酸酶检测试剂盒检测分化效果,茜素红染色观察矿化效果。发现30mW/cm2和40mW/cm2的超声对细胞产生促增殖作用,50mW/cm2抑制增殖;1.5MHz和1.7MHz的较低强度组均较对照组的ALP活性有增加;1.5MHz,40mW/cm2的超声组矿化效果较其他组好。研究结果表明,低强度的脉冲超声可以提高成骨细胞株MC3T3-E1增殖、分化和矿化能力,可能是防治骨质疏松的可行手段之一。
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| 关 键 词： | 低强度脉冲超声 成骨细胞 增殖 分化 矿化 |
Influence of LIPUS on Proliferation,Differentiation and Mineralization of MC3T3-E1 Cells |
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| WANG Ran,MA Jun,ZHAN Xin,XIN Min,WANG Jie,WANG Xiaowen,TANG Jintian,YUE Bingfei.Influence of LIPUS on Proliferation,Differentiation and Mineralization of MC3T3-E1 Cells[J].Science & Technology Review,2013,31(32):64-67. |
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| Authors: | WANG Ran MA Jun ZHAN Xin XIN Min WANG Jie WANG Xiaowen TANG Jintian YUE Bingfei |
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| Institution: | 1. Department of Biopharmaceuticals, Beijing University of Chinese Medicine, Beijing 100102, China;2. Department of Engineering Physics, Tsinghua University, Beijing 100084, China;3. Department of Laboratory Animal Quality Testing, National Institutes for Food and Drug Control, Beijing 100050, China |
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| Abstract: | The aim of the present study is to investigate the effectiveness of low-intensity pulsed ultrasound(LIPUS) on cell proliferation, differentiation and mineralizationusing of MC3T3-E1 cell line. The MC3T3-E1 cells were exposed to LIPUS with different parameters(frenquency and intensity). The cell proliferations, differentiation, and mineralization were determined by CCK-8 method, alkaline phosphatase detection kit, and alizarin red staining, respectively. The ultrasound intensity of 30mW/cm2 and 40mW/cm2 could promote cell proliferation, while 50mW/cm2 inhibit it. The lower intensity group under 1.5MHz and 1.7MHz present better alkaline phosphatase activity. The 1.5MHz and 40 mW/cm2 groups showed better mineralization effect than others. LIPUS can promote MC3T3-E1 on cell proliferation, differentiation and mineralization. LIPUS may act as a new method forprevention and treatment of osteoporosis. |
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