维药神香草总黄酮对卵清白蛋白致大鼠哮喘模型气道炎症的影响

 通过观察哮喘大鼠肺组织病理学改变、测定支气管肺泡灌洗液（BALF）细胞总数、淋巴细胞（Ly）、嗜酸性粒细胞（Eos）、中性粒细胞（Neu）的百分比以及肺组织和BALF中的转化生长因子-β1（TGF-β1），以探讨神香草总黄酮对哮喘大鼠气道炎症的影响，阐明维药神香草总黄酮抗哮喘的部分作用机制。试验中将大鼠随机分为正常对照组、哮喘模型组、氨茶碱阳性对照组、神香草总黄酮高、中、低剂量治疗组。采用卵清白蛋白（OVA）、氢氧化铝及百白破疫苗联合致敏和OVA生理盐水雾化激发的方法制备哮喘模型。测定BALF中细胞总数、Ly、Eos、Neu的百分比；采用酶联免疫吸附试验（ELISA）法检测肺组织及BALF中TGF-β1水平。结果显示，与模型组相比，各治疗组中，大鼠BALF中细胞总数、Ly、Eos、Neu的百分比、TGF-β1水平及肺组织中的TGF-β1水平均明显下降，差异有统计学意义（P<0.05）；神香草总黄酮各剂量组之间相互比较，BALF及肺组织中的以上指标差异均有统计学意义（P<0.05），呈剂量依赖性趋势。由此推测，维药神香草总黄酮可能通过抑制Ly、Eos、Neu等炎症细胞及细胞因子TGF-β1的分泌，减轻或改善哮喘的气道炎症。

Effect of Uighur Medicine Hyssopus officinalis L. Total Flavonoids on Airway Inflammation in Ovalbumin-induced Asthmatic Rats

The effect of the Uygur medicine Hyssopus officinalis L. total flavonoids on pathological changes in the lung tissue of asthmatic rats is observed and the total number of cells and the percentage of lymphocytes (Ly), eosinophils (Eos), neutrophils (Neu) in BALF, the level of TGF-β1 in BALF and the lung tissue are studied for the mechanism of its treatment of asthma. The rats were randomly divided into the normal control group, the asthma model group, the aminophylline treatment group, the Hyssopus total flavonoids groups of high, medium and low dose treatments. They were sensitized with OVA, Al(OH)3 and DPT vaccine and then were challenged with the inhalation of aerosolized OVA solution for preparation of the asthma model. The level of the lung tissue and BALF TGF-β1 were determined with ELISA, the total number of cells and the percentage of Ly, Eos, Neu in BALF were determined by light microscope. The results show that as compared with the asthma model group, the level of TGF-β1, the total number of cells and the percentage of Ly, Eos, Neu in BALF and the level of the lung tissue TGF-β1 were significantly lower in those of treated groups (P<0.05); among different dose treatment groups, the level of TGF-β1, the total number of cells and the percentage of Ly, Eos, Neu in BALF and the level of the lung tissue TGF-β1 were significantly reduced (P<0.05) with a dose-dependent trend in alleviating the airway inflammation of the asthma., Hyssopus officinalis L. total flavonoids may inhibit the secretion of TGF-β1, Ly, Eos, Neu, reducing the airway inflammation of the asthma.