甘遂半夏汤中甘遂甘草反药组合加减应用的急性毒性研究

 通过急性毒性实验比较甘遂半夏汤、甘遂半夏汤去炙甘草、甘遂半夏汤去醋甘遂、甘遂半夏汤去炙甘草醋甘遂、炙甘草、醋甘遂、炙甘草配醋甘遂毒性的大小。将18~20g的昆明小鼠随机分为空白组、甘遂半夏汤组、甘遂半夏汤去炙甘草组、甘遂半夏汤去醋甘遂组、甘遂半夏汤去炙甘草醋甘遂组、炙甘草组、醋甘遂组、炙甘草配醋甘遂组,空白组灌胃蒸馏水,其余各组灌胃给药,各组在均未做出LD₅₀的情况下,采用连续给药28d,即累计半数致死量的方法来进行毒性大小的评价。结果显示,炙甘草配醋甘遂组、炙甘草组及甘遂半夏汤组LD_50(n)的值分别为44.21、48.26、51.01g生药·kg^-1,其余各组连续给药28d仍未达到半数致死量。由此表明,甘遂半夏汤去掉方中甘遂甘草反药组合中的一味或两味没有显示出蓄积毒性,两味反药组合在复方中应用其LD_50(n)值有增加趋势。

Acute Toxicity of Gansuibanxia Decoction Plus-minus Gansui and Gancao Anti-drug Combination

Though comparing the toxicity of the Gansuibanxia soup, Gansuibanxia soup with Zhigancao removed, Gansuibanxia soup with Cugansui removed, Gansuibanxia soup with Zhigancao and Cugansui removed, and Zhigancao, Cugansui and the compatibility of Zhigancao and Cugansui, the toxicity of single herbs, anti-drug combinations, and the compound containing anti-drug combination is evaluated by dismantling the compound containing anti-drug combination of Gansui and Gancao. The Kunming male mice of 18-20g were randomly divided into a blank group, Gansuibanxia soup group, Gansuibanxia soup with Zhigancao removed group,Gansuibanxia soup with Cugansui removed group, Gansuibanxia soup with Zhigancao and Cugansui removed group, Zhigancao group, Cugansui group and the compatible group of Zhigancao and Cugansui. The blank group was administered with distilled water and other groups were administered with related drugs. For each group, LD₅₀ is obtained in this way: The drugs are continuously administered for 28 days, to cause half of the lethal to evaluate the toxicity. The results show that the values of LD_50(n) for the compatible group of Zhigancao and Cugansui, the Zhigancao group, the euphorbia and pinellia soup group are: 44.21, 48.26, 51.01g crude drug·kg^-1, and for other experimental groups, the half lethal dose is not reached in the case of the continuous administration for 28 days. It is suggested that the Gansuibanxia soup with Gansui and Gancao removed, the anti-drug combination or individually with Gansui or Gancao removed, alone do not show the cumulative toxicity, while for the two flavor combination of anti-drug application in the compound, the LD_50(n) value has an increasing trend.