| 赤子爱胜蚓(Eisenia foetida)内切型纤维素酶组分鉴定及纯化 |
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| 引用本文： | 武金霞,张永阔,田志鹏.赤子爱胜蚓(Eisenia foetida)内切型纤维素酶组分鉴定及纯化[J].河北大学学报(自然科学版),2011,31(1):63-68. |
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| 作者姓名： | 武金霞 张永阔 田志鹏 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
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| 摘 要： | 从赤子爱胜蚓(Eisenia foetida)匀浆液中提取出粗酶液,利用DNS法分析粗酶液中内切型纤维素酶(也称Cx酶)的活力.用刚果红染色法确定了赤子爱胜蚓含有3种内切型纤维素酶,将其分别称作Cx酶Ⅰ、Cx酶Ⅱ和Cx酶Ⅲ.经DEAE-Cellulose-52离子交换层析分离内切型纤维素酶,得到0.15～0.20 mo...
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| 关 键 词： | 赤子爱胜蚓 内切型纤维素酶 离子交换层析 刚果红染色 |
Identification and Purification of Endocellulases from Eisenia foetida |
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| WU Jin-xia,ZHANG Yong-kuo,TIAN Zhi-peng.Identification and Purification of Endocellulases from Eisenia foetida[J].Journal of Hebei University (Natural Science Edition),2011,31(1):63-68. |
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| Authors: | WU Jin-xia ZHANG Yong-kuo TIAN Zhi-peng |
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| Institution: | (College of Life Science,Hebei University,Baoding 071002,China) |
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| Abstract: | The crude enzyme solution from Eisenia foetida homogenate liquid was extracted,and the activity of endocellulases(also called Cx enzymes) was analyzed by DNS method.Three Cx enzymes were identified by Congo red staining,named as Cx enzyme Ⅰ,Cx enzyme Ⅱ and Cx enzyme Ⅲ respectively.DEAE-Cellulose-52 ion exchange chromatography were used to separate endocellulases,it was found that the effluent of 0.15～0.20 mol/L NaCl contained the active component.Cx enzyme Ⅰ and Cx enzyme Ⅱ were obtained by preparative electrophoresis,their molecular weights were 33 400 u and 28 800 u respectively,which determined by SDS-PAGE.The optimal temperature of endocellulases in the crude enzyme solution measured by DNS method was 40 ℃,and the optimal pH was pH 7.0. |
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| Keywords: | Eisenia foetida endocellulases ion exchange chromatography Congo red stainin |
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