| CeO2纳米颗粒对A549细胞的保护作用 |
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| 引用本文： | 申世刚,刘慧玲,王文颖,谷广其,周国强. CeO2纳米颗粒对A549细胞的保护作用[J]. 河北大学学报(自然科学版), 2011, 31(2): 160-166 |
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| 作者姓名： | 申世刚 刘慧玲 王文颖 谷广其 周国强 |
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| 作者单位： | 河北大学,化学与环境科学学院,河北省化学生物学重点实验室,河北,保定,071002 |
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| 基金项目： | 河北省科技厅重点资助项目 |
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| 摘 要： | 探讨了CeO2纳米颗粒对体外培养的人肺腺癌细胞A549的生物效应.使用扫描电子显微镜(SEM)对CeO2纳米颗粒进行了表征,采用噻唑蓝比色法测定了不同质量浓度CeO2纳米颗粒悬液对A549细胞活性的影响,对其抗氧化能力进行了检测,并且测定了30 nm CeO2作用24 h后细胞中的超氧化物歧化酶(SOD)和谷胱甘肽(G...
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| 关 键 词： | CeO2纳米颗粒 人肺腺癌(A549)细胞 生物效应 氧自由基 |
Protection Effects of CeO2 Nanoparticles on A549 Cells |
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| SHEN Shi-gang,LIU Hui-ling,WANG Wen-ying,GU Guang-qi,ZHOU Guo-qiang. Protection Effects of CeO2 Nanoparticles on A549 Cells[J]. Journal of Hebei University (Natural Science Edition), 2011, 31(2): 160-166 |
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| Authors: | SHEN Shi-gang LIU Hui-ling WANG Wen-ying GU Guang-qi ZHOU Guo-qiang |
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| Affiliation: | (Key Laboratory of Chemical Biology of Hebei Province,College of Chemistry and Environment Science,Hebei University,Baoding 071002,China) |
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| Abstract: | To investigate the biological effects of CeO2 nanoparicles on A549 human lung adenocarcinoma cells.CeO2 nanoparticles were characterized by scanning electron microscope(SEM).The viability effects of different concentrations of CeO2 nanoparticles suspension on A549 cells were tested with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.The antioxidant capacity was also determined.The activities of cellular superoxide dismutase(SOD) and glutathione(GSH) were determined after exposure to 30 nm CeO2 for 24 h.The results indicated that CeO2 nanoparticles could increase the survival rate of A549 cells and degrade oxidative stress caused by H2O2.With ability to eliminate oxygen free radicals,CeO2 nanoparticles had the potential protective effect towards oxidative damage of A549 cells. |
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| Keywords: | CeO2 nanoparticles human lung adenocarcinoma(A549) cells biological effects oxygen free radicals |
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