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| Evidence for the Existence of Cross-Linked Intermediates during Unfolding and Refolding of CK in UGGE | |
| 作者姓名： | 王希成 谢成 杨建 周海梦 |
| 作者单位： | WANG Xicheng，XIE Cheng，YANG JianZHOU Haimeng Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China |
| 摘 要： | IntroductionCreatine kinase ( CK) plays an important role incell energy metabolism1] .It catalyses thereversible transfer of a phosphoryl group fromATP to creatine generating phosphocreatine andADP.Owing to its importance in bioenergetics,CK has been extensively studied.There are fourisoforms of the enzyme:muscle type( MM) ,braintype ( BB) ,hybrid type ( MB) ,and mitochondriatype( Mi Mi) .Muscle- CK is a dimer composed of twoidentical subunits,both with relative molecularmass of4.3×… |
Evidence for the Existence of Cross-Linked Intermediates during Unfolding and Refolding of CK in UGGE |
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| Abstract: | |
| Keywords: | creatine kinase unfolding and refolding urea gradient gel electrophoresis intermediate disulfide bonds |
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