PP333对水稻幼苗过氧化物酶的调节

PP333处理后,苗高明显矮化,根长则表现为低浓度促进高浓度抑制,同时苗体内过氧化物酶(POD)活性及其同工酶谱带随之发生相应变化。统计分析的结果表明:苗高与POD活性呈显著负相关(r=-0.9325);根长与POD活性呈不显著负相关(r=-0.4216)。用外源GA_3逆转PP333的抑制作用时,POD活性下降,同工酶谱带着色变浅,C_3带几乎消失,用NAA处理未见有逆转PP333的抑制效应,同时POD活性提高,同工酶谱带增加两条(B_1、C_2带)。由此可以认为:PP333对植物生长的调节作用是通过对酶的影响而实现的。

REGULATION OF PP333 ON PEROXIDASE ACTIVITY AND ITS ISOZYME IN RICE SEEDLING

Control of rice seedling growth with PP333 and its relationship with the peroxidase activity and its isozyme were studied. The resultshowed that the plant height of seedling was significantly lower than that of the control after PP333 treatment. As to the growth of the root, low concentration of PP333 can promote the growth of the root, but high concentration will inhibit the root from growing up. The peroxidase activity and its isozymogram in seedling vary markedly with different treatments correspondingly. The plant height of rice seedling is negatively and significantly correlated to peroxidase activity (r= -0.9325*) . There was a tendency that the length of rice root was negatively correlated to peroxidase activity (r= -0.4216). Under the conditions of application of exogenous GA3, the retardant effect of PP333 was reversed. The peroxidase activity in rice seedling was reduced. The coloration of the peroxidase isozymogram varied lightly and the C3 band near the negative pole was almost in noncoloration. As the application of NAA, the retardant effect of PP333 could not be reversed and the peroxidase activity in rice seedling was enhanced, and it was found that there were two new bands (B1. C2) of isozymogram. It may be concluded that PP333 is possible through regulation on enzyme to control plant growth.