| 高效液相色谱法测定花生中黄曲霉素的含量 |
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| 引用本文： | 赵宁,郭玉梅,姚妍妍,许佳. 高效液相色谱法测定花生中黄曲霉素的含量[J]. 科技信息, 2009, 0(25): 44-44,29 |
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| 作者姓名： | 赵宁 郭玉梅 姚妍妍 许佳 |
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| 作者单位： | 华北煤炭医学院药学系,河北唐山063000 |
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| 摘 要： | 目的测出黄曲霉素在花生中的含量。方法采用高效液相谱（HPLC）法,使用Agilent1100色谱柱;在柱温30℃;乙腈-甲醇-水（1：1：2）为流动相;流速为1．0ml／min;荧光检测器;激发波长为360nm;发射波长为450nm的条件下测定。待测物经甲醇萃取及免疫亲合柱提取,内标法峰面积定量。结果黄曲霉素的色谱响应线性关系良好,方法重复性的RSD为0．38％,平均回收率为78．4％。结论该方法专属性强,灵敏度高,精密度和准确度好。
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| 关 键 词： | HPLC 黄曲霉素 花生 |
Determination of Aflatoxins in Peanut by HPLC Method |
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| ZHAO Ning,GUO Yu-mei,YAO Yan-yan,XU Jia. Determination of Aflatoxins in Peanut by HPLC Method[J]. Science, 2009, 0(25): 44-44,29 |
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| Authors: | ZHAO Ning GUO Yu-mei YAO Yan-yan XU Jia |
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| Affiliation: | (North China Coal Medical University, Tangshan Hebei ,063000,China) |
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| Abstract: | Objective To develop an HPLC method for the determination of Aflatoxins in nut. Methods The separation was performed on a DDS- C18 column （4.6×250 mm, 5 μm） using gradient etion with solution of acetonitrile-methano-water （1：1：2） as mobile phase; The flow rate was 1.0 ml/ min fluorescence detection excitation wavelength 360nm and emission wavelength 450 and the column temoerate was maintained at 30℃. Results Diosgenin was steady in methanol within 5 days and the insurument precision RSD was 0.061%, while the method precision RSD was 0.389%, The cuerage recovery RSD was 3.45% （n=5）. Conclusions The method is quick, aceurate, sensitive and simple with good. |
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| Keywords: | HPLC Aflatoxins Peanut |
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