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| 牛奶中硫氰酸盐高效液相色谱测定方法 | |
| 引用本文： | 赵文荣,李滢倩,李 刚,邴 炜.牛奶中硫氰酸盐高效液相色谱测定方法[J].北京工商大学学报(自然科学版),2013,31(1):43-45. |
| 作者姓名： | 赵文荣 李滢倩 李 刚 邴 炜 |
| 作者单位： | 1. 吉林农业大学食品科学与工程学院,吉林长春,130118 2. 吉林省产品质量监督检验院,吉林长春,130022 3. 吉林农业大学食品科学与工程学院,吉林长春 130118;吉林省产品质量监督检验院,吉林长春130022 |
| 摘 要： | 建立了高效液相色谱法分析牛乳中硫氰酸盐的方法.以戴安IonPac AS19(4 mm×250mm)阴离子色谱柱进行分离.二极管阵列检测器测定,流动相为0.05 mol/L Na2HP04溶液(pH值约为9.3),流速为0.8 mL/min,进样量10μL,检测波长218 nm.结果表明,牛奶中硫氰酸盐线性范围为5～100μg/mL,加标回收率为67.1%～101.8%,相对标准偏差(n=3)小于4.4%,最低检出限为0.5μg/mL. |
| 关 键 词： | 硫氰酸盐 阴离子色谱柱 液相色谱 |
High Performance Liquid Chromatographic Determination of Thiocyanate in Milk |
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| ZHAO Wen-rong,LI Ying-qian,LI Gang and BING Wei.High Performance Liquid Chromatographic Determination of Thiocyanate in Milk[J].Journal of Beijing Technology and Business University:Natural Science Edition,2013,31(1):43-45. | |
| Authors: | ZHAO Wen-rong LI Ying-qian LI Gang and BING Wei |
| Institution: | College of Food Science and Engineering, Jilin Agricultural University;Jilin Province Product Quality Supervision Test Institute;College of Food Science and Engineering, Jilin Agricultural University Jilin Province Product Quality Supervision Test Institute;College of Food Science and Engineering, Jilin Agricultural University Jilin Province Product Quality Supervision Test Institute |
| Abstract: | A high performance liquid chromatographic(HPLC) method was developed for determining thiocyanate in milk.HPLC analysis was performed on a Dionex IonPac AS19(4 mm×250 mm) anionic chromatography column with DAD detector,0.05 mol/L Na2HPO4 solution was used as mobile phase(pH 9.3) at a flow rate of 0.8 mL/min,sample loading amount was 10 μL,and detection wavelength was 218 nm.The method exhibited a limit of detection of 0.5 μg/mL and a linear range of 5-100 μg/mL.Spike recoveries of thiocyanate in milk powder and liquid milk at two levels ranged from 67.1%-101.8%,with RSDs(n=3) less than 4.4%. |
| Keywords: | thiocyanate Anionic chromatography column high performance liquid chromatography |
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