| 海带中脱落酸的分离纯化及高效液相色谱分析 |
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| 引用本文： | 苗钧魁,李铁松,王长海.海带中脱落酸的分离纯化及高效液相色谱分析[J].烟台大学学报(自然科学与工程版),2009,22(4):295-298. |
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| 作者姓名： | 苗钧魁 李铁松 王长海 |
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| 作者单位： | 1. 大连理工大学,环境与生命科学学院,辽宁,大连116024
2. 大连理工大学,环境与生命科学学院,辽宁,大连116024;辽宁师范大学,生命科学院,辽宁,大连,116029 |
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| 基金项目： | 国家高技术研究发展计划("十五"-863计划) |
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| 摘 要： | 探索了海带（Laminaria japonica）脱落酸的分离纯化条件,获得最适分离纯化方法为：常温组织匀浆,80%甲醇溶液浸提,乙酸乙酯萃取,硅胶脱色和纯化.在此基础上,利用反向高效液相色谱的方法对样品进行定性定量分析,结果表明样品中含有脱落酸,含量为30.5-60.5μg/kg.这为海藻脱落酸的分离纯化及其活性研究奠定了基础.
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| 关 键 词： | 海带 脱落酸 分离纯化 高效液相色谱 |
Isolation and Purification of ABA from Laminaria japonica Aresch and Determination by HPLC |
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| MIAO Jun-kui,LI Tie-song,WANG Chang-hai.Isolation and Purification of ABA from Laminaria japonica Aresch and Determination by HPLC[J].Journal of Yantai University(Natural Science and Engineering edirion),2009,22(4):295-298. |
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| Authors: | MIAO Jun-kui LI Tie-song WANG Chang-hai |
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| Institution: | MIAO Jun-kui, LI Tie-song, WANG Chang-hai ( 1. School of Environmental and Biological Engineering, Dalian University of Technology, Dalian 116024, China ; 2. College of life sciences, Liaoning Normal University, Dalian, 116029, China) |
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| Abstract: | A method to determine endogenetic abscisic acid（ABA） in kelp is studied.The optimal process includes extration with 80% methanol,secondary extraction with ethyl acetate from the former extration,decoloration and purification with silica gel.Reverse-phase high performance liquid chromatography（RP-HPLC） and liquid chromatography-mass spectrometry（LC-MS） are employed in the qualitative and quantitative analysis for ABA in the sample.The result shows that abscisic acid does exist in kelp and its content is 30. 5 - 60.5μg/kg. The research can be used as reference for the further investigation on biological activity of ABA in kelp. |
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| Keywords: | Laminaria japonica abscisic acid isolation and purification HPLC |
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