| 套式PCR检测淋巴细胞脉络丛脑膜炎病毒(LCMV)方法应用 |
| |
| 引用本文： | 邢进,肖镜,王吉,贺争鸣.套式PCR检测淋巴细胞脉络丛脑膜炎病毒(LCMV)方法应用[J].实验动物科学,2008,25(5):53-55. |
| |
| 作者姓名： | 邢进 肖镜 王吉 贺争鸣 |
| |
| 作者单位： | 中国药品生物制品检定所实验动物质量检测室,北京,100050 |
| |
| 摘 要： | 目的检测目前小鼠和地鼠中淋巴细胞脉络丛脑膜炎病毒的感染情况。方法采用套式PCR方法对小鼠和地鼠脑及小鼠脏器进行扩增,检测LCMV基因;同时对血清进行ELISA试验,将两种方法的结果进行比较。结果从小鼠的脾脏和肾脏中检测到NP基因的存在,检出率2/148;ELISA检出率5/148。结论PCR与ELISA检测方法相结合,准确率高,证明目前小鼠中存在LCMV的感染。
|
| 关 键 词： | 淋巴细胞脉络丛脑膜炎病毒 套式PCR检测 |
| 收稿时间： | 2008-10-28 |
Application of Assay for The Detection of LCMV Nested Polymerase Chain Reaction |
| |
| XING Jin,XIAO Jing,WANG Ji,HE Zheng-ming.Application of Assay for The Detection of LCMV Nested Polymerase Chain Reaction[J].Shiyan Dongwu Kexue,2008,25(5):53-55. |
| |
| Authors: | XING Jin XIAO Jing WANG Ji HE Zheng-ming |
| |
| Institution: | ( Department of The Quality of Laboratory Animal Testing, National Institute for The Control of Pharmaceutical ＆ Biological Products, Beijing 100050, China ) |
| |
| Abstract: | Objective Monitoring infection currently. Methods Using nested-PCR method in Lymphocytic Choriomeningitis Virus in mice and Golden hamster mice and Golden hamster to detect the presence of LCMV gene. At the same time to take the serum ELISA test, the results of the two methods were compared. Results The PCR results showed existence of NP gene in spleen and kidney of mice. PCR positive rate is 2/148, ELISA is 5/148. Conclusion Combining of PCR and ELISA method can improve accuracy rate and prove currently exist in mice LCMV infection. |
| |
| Keywords: | LCMV Nested-PCR test |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《实验动物科学》浏览原始摘要信息 |
| 点击此处可从《实验动物科学》下载免费的PDF全文 |
|
