| 蓖麻蚕蛹mtDNA的限制性内切酶图谱 |
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| 引用本文： | 凌建华,陈元霖.蓖麻蚕蛹mtDNA的限制性内切酶图谱[J].厦门大学学报(自然科学版),1993,32(5):641-646. |
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| 作者姓名： | 凌建华 陈元霖 |
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| 作者单位： | 厦门大学细胞生物学研究室
(凌建华)，厦门大学细胞生物学研究室(陈元霖) |
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| 摘 要： | 采用碱变性法制备蓖麻蚕蛹ｍｔＤＮＡ，经九种限制性内切酶单酶和双酶酶解后，琼脂糖凝胶电泳检测酶切位点数和酶切片段长度。根据片段的大小确定消失片段与新生片段关系，通过对片段重叠、拼接、判断各片段邻近位置，从而构建了９种限制性内切酶、共２４个酶切位点蓖麻蚕蛹ｍｔＤＮＡ酶切图谱。
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| 关 键 词： | 蓖麻蚕 蚕蛹 mtDNA 限制性内切酶 |
Restriction Endouclease Cleavage Maps of MtDNA from the Pupas of Eri Silkworms |
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| Ling Jianhua Chen Yuanltn.Restriction Endouclease Cleavage Maps of MtDNA from the Pupas of Eri Silkworms[J].Journal of Xiamen University(Natural Science),1993,32(5):641-646. |
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| Authors: | Ling Jianhua Chen Yuanltn |
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| Institution: | Inst. of Cell Biol. |
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| Abstract: | Mitochondrial DMA (mtDNA) form the pupas of Eri silkworms wasprepared by the alkaline lysis procedure, and was digested by nine kinds of restriction; fragments were checked by agarose gel electrophoresis. According to analysis of single and double enzyme cleavage, the map of restriction enzyme was constructed. |
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| Keywords: | EH silkworm MtDNA Restriction map Pupa |
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