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| 水稻花药发育的细胞形态学精细分期 |
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| 引用本文： | 李贵节 .水稻花药发育的细胞形态学精细分期 [J].重庆师范大学学报(自然科学版),2011(3):56-59. |
| 作者姓名： | 李贵节 |
| 作者单位： | 重庆教育学院生物与化学工程系; |
| 摘 要： | 本研究通过观察籼型杂交水稻"川优9527"的花粉和药室壁的发育,对幼穗花药从雄蕊原基形成至花粉成熟的全过程进行了分期。选用不同发育时期的水稻幼穗(长度约1.0~10.0 mm),以石蜡切片技术分别制作其花药装片,置于光学显微镜(N ikon DS-R i1-U2)下观察并拍照。根据细胞及组织形态特征的显著变化,将花药的发育进程精细划分为:孢原细胞期、二层药室壁期、三层药室壁期、花粉母细胞形成期(以上4期对应小孢子母细胞形成期)、花粉母细胞减数分裂初期、花粉母细胞减数分裂中期、花粉母细胞二分体时期、花粉母细胞四分体时期(以上4期对应小孢子母细胞减数分裂期)、小孢子早期、小孢子中期、小孢子晚期、二胞花粉时期、成熟花粉时期等13个阶段。 |
| 关 键 词： | 水稻 川优9527 花药 发育分期 花粉母细胞 药室壁 |
Fine Division of Rice Anther Development by Cytological Morphology |
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| LI Gui-jie .Fine Division of Rice Anther Development by Cytological Morphology [J].Journal of Chongqing Normal University:Natural Science Edition,2011(3):56-59. |
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| Authors: | LI Gui-jie |
| Institution: | LI Gui-jie(Dept.of Biological and Chemical Engineering,Chongqing Education College,Chongqing 400067,China) |
| Abstract: | A cytological and morphological study was conducted on the anther of a hybrid rice(Oryza Sativa L.indica) strain,Chuan You 9527.To study the growth procedures of pollen and anther wall,their development processes from stamen primordia initiation to pollen maturation were observed and divided into stages.Rice spikelets ranging from approx.1.0 millimeter to approx.10.0 millimeter were chosen;loading films of anthers were prepared by using the techniques of paraffin section,and they were observed and then phot... |
| Keywords: | Oryza Sativa L Chuan You 9527 anther development stages pollen mother cell anther wall |
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