| 紫茉莉不同花色植株遗传变异的ISSR分析 |
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| 引用本文： | 葛方兰,周小奎.紫茉莉不同花色植株遗传变异的ISSR分析[J].安徽大学学报(自然科学版),2009,33(3). |
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| 作者姓名： | 葛方兰 周小奎 |
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| 作者单位： | 1. 四川师范大学,生命科学学院,四川,成都,610066
2. 四川师范大学,生命科学学院,四川,成都,610066;四川大学,建筑与环境学院,四川,成都,610064 |
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| 基金项目： | 四川师范大学校级重点基金资助项目(061k008) |
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| 摘 要： | 采用简单序列重复区间扩增ISSR分子标记技术对分布于四川省成都市的紫茉莉(Mirabilis jalapaL.)3种不同花色群体进行了遗传变异分析.从100条引物筛选出5条引物进行PCR扩增,共扩增出67条清晰条带,其中62条条带具多态性.紫茉莉的等位多态性位点百分数(PPB)为92.54%,观测等位基因数(Na)为1.9254,有效等位基因数(Ne)为1.2354,Nei的基因多样性指数(H)为0.1562,Shannon指数(I)为0.2633.3种花色的遗传一致度在0.9054~0.9620之间,遗传距离在0.0388~0.0994之间,其中白色与黄色的遗传距离最小(0.0388),表现出最近的亲缘关系,黄色与紫色的遗传距离稍大(0.0994),亲缘关系稍远.紫茉莉的遗传变异主要存在于群体内,不同花色群体间的基因流(Nm)为1.279.
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| 关 键 词： | 紫茉莉 遗传分化 ISSR |
ISSR analysis of genetic variation of Mirabilis jalapa L. with different color flowers |
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| GE Fang-lan,ZHOU Xiao-kui.ISSR analysis of genetic variation of Mirabilis jalapa L. with different color flowers[J].Journal of Anhui University(Natural Sciences),2009,33(3). |
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| Authors: | GE Fang-lan ZHOU Xiao-kui |
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| Institution: | 1.College of Life Science;Sichuan Normal University;Chengdu 610066;China;2.College of Architecture and Environment;Sichuan University;Chengdu 610064;China |
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| Abstract: | Mirabilis jalapa L.belong to the Mirabilis genus,which was classified in the family Nyctaginaceae.Inter-simple sequences repeats(ISSR) markers were used to analysis the genetic variation among 3 colonys with different color flowers.Of the 100 primers,5 produced highly reproducible ISSR bands.These primers were carried out on 3 colonys of M.jalapa with purple,white and yellow flowers from Chengdu City,Sichuan Province.High genetic variation was revealed.67discernable DNA fragments were with 62(92.54 %)being ... |
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| Keywords: | Mirabilis jalapa L genetic variation ISSR |
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