| Purification and Characterization of an Antibacterial Protein from the Cultured Mycelia of Cordyceps sinensis |
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| 作者姓名： | HU Zheng~ YE Maoqing~ XIA Liqiu~ TU Wenjuan~ LI Liang~ ZOU Guolin~ . College of Life Sciences Wuhan University Wuhan Hubei China . Key Laboratory of Industrial Microbiology of Hubei Province Hubei University of Technology Wuhan Hubei China . College of Life Sciences Hunan Normal University Changsha Hunan China |
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| 作者单位： | HU Zheng~ 1，2，YE Maoqing~1，XIA Liqiu~3，TU Wenjuan~2，LI Liang~2，ZOU Guolin~ 1 1. College of Life Sciences，Wuhan University，Wuhan 430072，Hubei，China; 2. Key Laboratory of Industrial Microbiology of Hubei Province，Hubei University of Technology，Wuhan 430068，Hubei，China; 3. College of Life Sciences，Hunan Normal University，Changsha 410081，Hunan，China |
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| 基金项目： | 中国科学院资助项目;湖北省自然科学基金 |
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| 摘 要： | 0 IntroductionAlnattie mdicfrroobmiala p wriodteei nvsar iheatdy boefe linv ifnogun odr gaanndis ismos--Bacteria1], fungi2 ,3], plants4]and ani mals5].Those proteins displayed a wide spectrumof anti mi-crobial activity against different species of viruses ,bacteria andfungi .Over the past few years ,several anti microbialpeptides and proteins were foundinfungus ,such asAFP fromAspergillus giganteus6], Anafp fromAspergillus niger7], Zygocin fromthe yeastZy-gosaccharomyces bailii8],an…
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| 关 键 词： | 冬虫夏草 抗菌蛋白 分离 鉴定 CSAP |
| 文章编号： | 1007-1202（2006）03-0709-06 |
| 收稿时间： | 2005-05-10 |
Purification and characterization of an antibacterial protein from the cultured mycelia ofCordyceps sinensis |
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| Hu Zheng,Ye Maoqing,Xia Liqiu,Tu Wenjuan,Li Liang,Zou Guolin.Purification and Characterization of an Antibacterial Protein from the Cultured Mycelia of Cordyceps sinensis[J].Wuhan University Journal of Natural Sciences,2006,11(3):709-714. |
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| Authors: | Hu Zheng Ye Maoqing Xia Liqiu Tu Wenjuan Li Liang Zou Guolin |
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| Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, China;(2) Key Laboratory of Industrial Microbiology of Hubei Province, Hubei University of Technology, 430068 Wuhan, Hubei, China;(3) College of Life Science, Hunan Normal University, 410081 Changsha, Hunan, China |
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| Abstract: | An antibacterial protein was isolated from the cultured mycelia ofCordyceps sinensis, and was designated asCordyceps sinensis Antibacterial Protein (CSAP). CSAP was single-chained, with an apparent molecular mass of 35×103 revealed by SDS-PAGE and a novel hydrophobic N-terminal sequence N-ALATQHGAP. The antimicrobial assays showed CSAP could
inhibit the growth of Gram-positive and Gram-negative bacteria but no significant inhibition against fungi or yeasts. Furthermore,
the antibacterial activity of CSAP was not bactericidal but bacteriostatic. It was the first time that an antibacterial protein
was described in theCordyceps species, which might involve in the chemical defense mechanism of the hosts.
Foundation item: Supported by the National Natural Science Foundation of China (39770200) and the Natural Science Foundation of Hubei Province
(2004ABA228)
Biography: HU Zheng (1964-), Male, Associate professor, research direction: biochemistry. |
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| Keywords: | Cordyceps species antimicrobial protein isolation identification membrane-activity proteins |
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