| 日本血吸虫Sj23与IL-12多价DNA疫苗的构建 |
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| 引用本文： | 卜玲毅,石佑恩,甘燕.日本血吸虫Sj23与IL-12多价DNA疫苗的构建[J].华中科技大学学报(自然科学版),2004,32(2):80-82. |
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| 作者姓名： | 卜玲毅 石佑恩 甘燕 |
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| 作者单位： | 华中科技大学,同济医学院寄生虫学教研室,湖北,武汉,430030;华中科技大学,同济医学院寄生虫学教研室,湖北,武汉,430030;华中科技大学,同济医学院寄生虫学教研室,湖北,武汉,430030 |
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| 摘 要： | 构建能共同表达日本血吸虫23kDa膜抗原(Sj23)与细胞因子IL-12的多价DNA疫苗．RT-PCR的方法从日本血吸虫成虫获得Sj23的基因片段，克隆到载体pVIVO2-mIL12／mcs上，进行酶切和测序鉴定．采用免疫荧光的方法检测多价DNA疫苗pVIVO2-Sj23-IL12在小鼠骨骼肌细胞的表达．成功地构建了能共同表达日本血吸虫23kDa膜抗原(Sj23)与细胞因子IL-12的多价DNA疫苗．此多价DNA疫苗在免疫小鼠肌细胞的细胞膜和细胞浆均获得了Sj23的表达．
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| 关 键 词： | 日本血吸虫 23kDa膜蛋白 多价DNA疫苗 克隆 表达 |
| 文章编号： | 1671-4512(2004)02-0080-03 |
| 修稿时间： | 2003年3月28日 |
Construction of the multivalent DNA vaccine of schistosoma japonicum Sj23 and cytokine IL-12 and its identification |
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| Bu Lingyi Shi Youen Gan YanDr., Tongji Medical College,Huazhong Univ. of Sci. & Tech.,Wuhan ,China..Construction of the multivalent DNA vaccine of schistosoma japonicum Sj23 and cytokine IL-12 and its identification[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2004,32(2):80-82. |
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| Authors: | Bu Lingyi Shi Youen Gan YanDr Tongji Medical College Huazhong Univ of Sci & Tech Wuhan China |
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| Institution: | Bu Lingyi Shi Youen Gan YanDr., Tongji Medical College,Huazhong Univ. of Sci. & Tech.,Wuhan 430030,China. |
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| Abstract: | To construct multivalent DNA vaccine coexpressing Sj2 3 and cytokine IL-12, DNA fragment encoding the Sj23 gene of schistosoma japonicu m was obtained from the adult worm by RT-PCR. The gene was cloned into expresse d plasmid pVIVO2-mIL12/mcs, and identified by restriction analysis and sequence . The expression of Sj23 in muscle tissue was determined by Immunofluorescence a ssay. Multivalent DNA vaccine which co-expresses Sj23 and cytokine IL-12 was s uccessfully constructed. Sj23 was expressed on the membrane and in the plasma of muscle cells of the mice immunized with pVIVO2-Sj23-IL-12. |
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| Keywords: | Schisosoma japonicum 23 kDa membrane protei n multivalent DNA vaccine cloning expression |
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