5个温郁金居群遗传多样性的ISSR分析

应用ISSR标记技术对瑞安、乐清和平阳三个地区5个居群的温郁金(Curcuma wenyujin)遗传多样性进行分析,筛选出6个引物用于温郁金5个居群的50个个体ISSR-PCR扩增,共检测到42个结合位点多态位点34个,多态位点百分率是80.95%.总的基因多样度平均值为0.239 5,居群内的基因多样度为0.161 8,居群间的基因多样性为0.0777.基因分化系数在0.019 1⁰.9414之间,平均值为0.324 5,其中居群间的遗传变异占总遗传变异的32.45%,居群内的遗传变异占总变异的67.55%.温郁金有着较高的遗传多样性水平,且居群内发生了较高的遗传分化.基因流系数Nm为1.041 0,表明温郁金居群间的基因交流也比较多.

The genetic diversity of the five populations Curcuma wenyujin by ISSR analysis

The genetic diversity of five populations of Curcuma wenyujin, a species endemic to Ruian, Yueqing Pingyang and, was analyzed using inter simple sequence repeat （ ISSR ） . A total of 174 6 bands and 42 loci were amplified from 5 populations by 6 informative and reliable primers of 40 primers. Of them, 34 loci were polymorphic, which accounted for 80.95%. Genetic diversity was revealed, Ht =0.239 5, Hs =0.1618, Dst=0.077 7 and GST =0.324 5. The study shows that there has a middle degree of genetic differentiation occurred among Curcuma wenyujin populations and a high genetic variation level beyond the populations of Curcuma wenyujin. Thus, Nm=1.041 0 means genetic was more keep in touch with each other among the population of Curcuma wenyujin.