外源有机碳浓度对藻菌关系及氮磷去除的影响

采用实验室一次培养法,以葡萄糖为有机碳源,对地衣芽孢杆菌和丝藻进行单独及混合培养,研究了外源有机碳浓度对藻菌相互关系及氮磷去除效率的影响。结果表明,当水体中葡萄糖质量浓度不高于20mg/L时,地衣芽孢杆菌能显著促进丝藻的生长。尤其当葡萄糖质量浓度为20mg/L时,藻菌体系叶绿素荧光强度最大可达5227,较纯丝藻体系相应值提高约15%;当葡萄糖质量浓度为50 mg/L时,藻菌体系对水体中NH_4~+-N和PO_4~(3-)-P的去除率分别达到27%和60%,显著高于纯丝藻及纯菌体系相应值;当葡萄糖质量浓度高于50mg/L时,藻菌体系和纯丝藻体系对氮磷的去除率无显著差异;当葡萄糖质量浓度高于100mg/L时,地衣芽孢杆菌明显抑制丝藻的生长。

Effects of exogenous organic carbon concentration on the algae-bacteria relationship and removal of nitrogen and phosphorus

In a batch culture experiment,different concentrations of glucose was used to study the effects of exogenous carbon source on the algae-bacteria relations and removal efficiency of nitrogen and phosphorus by Bacillus licheniformis and Ulothrix sp. separately or co-cultured. The results show that Bacillus licheniformis can significantly promote the growth of Ulothrix sp. at a glucose concentration of less than 20 mg/L. At the glucose concentration of 20 mg/L, chlorophyll fluorescence intensity of Ulothrix sp. can reach the maximum of 5227 , which is 15% higher than that of the monoculture of algae. When the concentration of glucose is 50 mg/L, the removal efficiency of nitrogen and phosphorus in algal-bacteria system is 27% and 60%, respectively, which are significantly higher than those in monoculture of algae or bacteria. When the concentration of glucose is higher than 50 mg/L, there is no significant difference in nitrogen and phosphorus removal efficiency between the mix-culture and monoculture. Bacillus licheniformis inhibits growth of Ulothrix sp. at glucose concentrations above 100 mg/L.