| 自制核心链霉亲和素的性质 |
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| 引用本文： | 马勇杰,高峰,古宏晨. 自制核心链霉亲和素的性质[J]. 上海师范大学学报(自然科学版), 2004, 33(3): 67-71 |
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| 作者姓名： | 马勇杰 高峰 古宏晨 |
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| 作者单位： | 上海交通大学,纳米科学与技术工程中心,上海,200030;上海交通大学,纳米科学与技术工程中心,上海,200030;上海交通大学,纳米科学与技术工程中心,上海,200030 |
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| 基金项目： | 国家863计划项目(2002AA302210). |
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| 摘 要： | 在实验室分离和纯化链霉亲和素的基础上对其性质进行了鉴定．在SDS-PAGE中链霉亲和素只有一条带,单体的分子量为14500Da,链霉亲和素的相对分子量约为58000Da;其等电点为5．9～6．2;结合生物素的活性为15．7U／mg．结果表明自制链霉亲和素为核心链霉亲和素,在纯度、活性等方面达到了理想的效果．
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| 关 键 词： | 链霉亲和素 生物素 聚丙烯酰胺凝胶电泳 等电聚焦电泳 |
| 文章编号： | 1000-5137(2004)03-0067-05 |
| 修稿时间： | 2004-02-17 |
A study on the characteristics of self-purified core streptavidin |
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| MA Yong-jie,GAO Feng,GU Hong-chen. A study on the characteristics of self-purified core streptavidin[J]. Journal of Shanghai Normal University(Natural Sciences), 2004, 33(3): 67-71 |
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| Authors: | MA Yong-jie GAO Feng GU Hong-chen |
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| Abstract: | On the basis of previous isolation and purification of Streptavidin(SA) , the authors identify its characteristics. The molecular weight of SA was about 58000 Dalton by SDS-PAGE and its isoelectric point was 5.9-6.2 by IEF-PAGE. The averaged biotin - binding activity was 15.7U/mg. The results showed the SA purified by authors is core Streptavidin, which brings a-bout ideal effect as far as purity and activity is concerned and is thus reliable for immunological application and can be used as the substitute for imported SA. |
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| Keywords: | streptavidin biotin SDS-PAGE IEF-PAGE |
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