| The Effect of Cyclic Stretching on Matrix Production, Mineralization and Differentiation of Osteoblasts |
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| 作者姓名： | 秦建 TANG LILING Wang Yuanliang Gu Li |
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| 作者单位： | Bioengineering College，Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education，Chongqing University Chongqing 400044，P.R.China |
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| 基金项目： | grantsfromNationalNaturalScienceFoundationofChina (19872 0 80 ) |
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| 摘 要： | A four-point bending apparatus is used to investigate the effects of stretching on collagen synthesis, mineralization and differentiation of osteoblasts. Cells are stretched at 1500με for 24 hours. The responses of osteoblasts to mechanical signal of physiological stretching are evaluatedfrom three aspects: collagen production, extracellular inorganic calcium secretion and ALP activity. The results show that osteoblasts decrease the collagen synthesis, calcium secretion and ALP activity compared to the control cells (65.82 %, 73.51%, 48.10 96 respectively ), confirming that cyclic stretching at 1500με inhabits the ohvsiological activity of osteoblasts.
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| 关 键 词： | 成骨细胞 机械拉伸 矿化 胞间质合成 四点弯曲装置 物理仿真 |
The Effect of Cyclic Stretching on Matrix Production, Mineralization and Differentiation of Osteoblasts |
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| TANG LILING,Wang Yuanliang,Gu Li.The Effect of Cyclic Stretching on Matrix Production, Mineralization and Differentiation of Osteoblasts[J].High Technology Letters,2003,9(3):33-35. |
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| Authors: | TANG LILING Wang Yuanliang Gu Li |
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| Abstract: | A four-point bending apparatus is used to investigate the effects of stretching on collagen synthesis, mineralization and differentiation of osteoblasts. Cells are stretched at 1500 με for 24 hours. The responses of osteoblasts to mechanical signal of physiological stretching are evaluated from three aspects: collagen production, extracellular inorganic calcium secretion and ALP activity. The results show that osteoblasts decrease the collagen synthesis, calcium secretion and ALP activity compared to the control cells (65.82%,73.51%,48.10% respectively), confirming that cyclic stretching at 1500 με inhabits the physiological activity of osteoblasts. |
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| Keywords: | osteoblast mechanical stretching matrix synthesis differentiation mineralization |
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