| 以六氨合钴(Ⅲ)为探针的DNA共振光散射分析 |
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| 引用本文： | 李正平,李艳坤,王愈聪,赵容超.以六氨合钴(Ⅲ)为探针的DNA共振光散射分析[J].河北大学学报(自然科学版),2004,24(6):611-614. |
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| 作者姓名： | 李正平 李艳坤 王愈聪 赵容超 |
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| 作者单位： | 河北大学,化学与环境科学学院,河北,保定,071002 |
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| 摘 要： | 在pH为1.81～4.1的Britton＿Robinson(BR)缓冲溶液中,Co(NH3)6]3+与脱氧核糖核酸(DNA)相互作用并使其共振光散射强烈的增强.在342.0nm处增强的共振光散射(RLS)强度与DNA的质量浓度在0.01～7.0mg/L范围内呈良好的线性关系.据此建立了操作简便、灵敏度高的测定痕量DNA的新方法.该方法用于检测小牛胸腺DNA,检出限达到1.6μg/L级.
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| 关 键 词： | 共振光散射 脱氧核糖核酸 六氨合钴(Ⅲ)离子 |
| 文章编号： | 1000-1565(2004)06-0611-04 |
| 修稿时间： | 2004年4月5日 |
Resonance Light Scattering Analysis of DNA by Using Cobaltic Hexammonate Ion as Probe |
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| LI Zheng-ping,LI Yan-kun,WANG Yu-cong,ZHAO Rong-chao.Resonance Light Scattering Analysis of DNA by Using Cobaltic Hexammonate Ion as Probe[J].Journal of Hebei University (Natural Science Edition),2004,24(6):611-614. |
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| Authors: | LI Zheng-ping LI Yan-kun WANG Yu-cong ZHAO Rong-chao |
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| Abstract: | In a Britton-Robinson (BR) buffer of pH 1.81～4.1, the interaction of Co(NH_3)_6]~(3+) with DNA results in strong enhancement of resonance light scattering (RLS) intensity. Ander the optical condition, the enhanced RLS intensity at 342.0 nm was proportional to the concertration of DNA in the range of 0.01～(7.0 mg/L.) A sensitive and convenient new analysis method for microdetermination of DNA was accordingly established. The limit of dertermination was 1.6 μg/L (3σ) for Calf thymus DNA (ctDNA). |
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| Keywords: | resonance light scattering deoxyribonucleic acid cobaltic hexammonate ion |
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