A comparative study of proteoglycans from bovine lung,trachea, tracheal mucosa,and aorta

Summary Proteoglycans were isolated from bovine lung, trachea, tracheal mucosa, and aorta by dissociative extraction with 4M guanidinium hydrochloride. Fractionation of these tissue extracts by cesium chloride density centrifugation and gel chromatography allowed the isolation from each extract of a high molecular weight fraction consisting mainly of proteochondroitin sulfate and hyaluronic acid.Acknowledgment. This work was supported by a National Heart, Lung, and Blood Institute grant to K. Ehrlich (HL21617).