| 棉花黄萎病菌遗传转化载体构建和农杆菌转化 |
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| 引用本文： | 彭姗,李静,杜娟,李晖.棉花黄萎病菌遗传转化载体构建和农杆菌转化[J].石河子大学学报,2007,25(1):34-38. |
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| 作者姓名： | 彭姗 李静 杜娟 李晖 |
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| 作者单位： | 石河子大学农学院植物保护系，石河子大学农学院植物保护系，石河子大学农学院植物保护系，石河子大学农学院植物保护系 新疆石河子832003，新疆兵团绿洲生态农业重点实验室，新疆石河子832003，新疆石河子832003，新疆石河子832003，新疆石河子832003，新疆兵团绿洲生态农业重点实验室，新疆石河子832003 |
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| 摘 要： | 载体构建是建立真菌遗传转化体系的基础。本研究以pCAMBIA1305.1双元载体为基本骨架,构建棉花黄萎病菌遗传转化T-DNA质粒双元载体,通过限制性内切酶酶切、补平、连接以及去磷酸化等措施将pCAMBIA1305.1上Hygromycin抗性基因的CaMV 35S启动子替换成构巢曲霉的trpC启动子。经大肠杆菌转化后对转化子进行酶切验证,并将新构建的载体1305-3分别转入农杆菌菌株LBA4404和EHA105。为农杆菌介导的黄萎病菌遗传转化体系的建立和优化提供了存在于不同农杆菌菌株中的供试载体。
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| 关 键 词： | 载体构建 农杆菌介导的转化 黄萎病菌 棉花 |
| 文章编号： | 1007-7383（2007）01-0034-05 |
| 收稿时间： | 2006-10-16 |
| 修稿时间： | 2006年10月16 |
Construction of Vector for Genetic Transformation of Cotton Verticillium dahliae |
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| PENG Shan,LI Jing,DU Juan,LI Hui.Construction of Vector for Genetic Transformation of Cotton Verticillium dahliae[J].Journal of Shihezi University(Natural Science),2007,25(1):34-38. |
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| Authors: | PENG Shan LI Jing DU Juan LI Hui |
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| Abstract: | Construction of binary vector is the basis for agrobacterium-mediated transformation of fungi.Binary vector used for agrobacterium-mediated transformation of Verticillium dahliae was constructed with pCAMBIA1305.1 as the backbone.CaMV 35S promotor of Hygromycinin pCAMBIA1305.1 was replaced by trpC promotor of aspergillus nidulans by procedures such as digestion with restriction enzyme,dephosphatasing with BCIP,and lingation with T4 lingase etc.The new constructed vector,1305-3,was transformed to the two agrobacterium strains,LBA4404 and EHA105,respectively.They were used to provide different types of vector in different types of agrobacterium strain for further genetic transformation of Verticillium dahliae and optimizing the transformation condition. |
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| Keywords: | vector construction agrobacterium-mediated transformation Verticillium dahliae cotton |
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