Dopamine receptor 1 localizes to neuronal cilia in a dynamic process that requires the Bardet-Biedl syndrome proteins |
| |
Authors: | Jacqueline?S?Domire Jill?A?Green Kirsten?G?Lee Andrew?D?Johnson Candice?C?Askwith Email author" target="_blank">Kirk?MykytynEmail author |
| |
Institution: | (1) Department of Pharmacology, College of Medicine, The Ohio State University, 5034 Graves Hall, 333 West 10th Ave, Columbus, OH 43210, USA;(2) Department of Neuroscience, College of Medicine, The Ohio State University, Columbus, OH 43210, USA;(3) The National Heart, Lung and Blood Institute’s Framingham Heart Study, Center for Population Studies, Framingham, MA 01702, USA; |
| |
Abstract: | Primary cilia are nearly ubiquitous cellular appendages that provide important sensory and signaling functions. Ciliary dysfunction
underlies numerous human diseases, collectively termed ciliopathies. Primary cilia have distinct functions on different cell
types and these functions are defined by the signaling proteins that localize to the ciliary membrane. Neurons throughout
the mammalian brain possess primary cilia upon which certain G protein-coupled receptors localize. Yet, the precise signaling
proteins present on the vast majority of neuronal cilia are unknown. Here, we report that dopamine receptor 1 (D1) localizes
to cilia on mouse central neurons, thereby implicating neuronal cilia in dopamine signaling. Interestingly, ciliary localization
of D1 is dynamic, and the receptor rapidly translocates to and from cilia in response to environmental cues. Notably, the
translocation of D1 from cilia requires proteins mutated in the ciliopathy Bardet-Biedl syndrome (BBS), and we find that one
of the BBS proteins, Bbs5, specifically interacts with D1. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|