| 荧光分析法测定肉类食品中诺氟沙星的残留 |
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| 引用本文： | 蔡其洪,许桂芬,阮丽琴,李春来.荧光分析法测定肉类食品中诺氟沙星的残留[J].沈阳大学学报,2012,24(5):19-22. |
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| 作者姓名： | 蔡其洪 许桂芬 阮丽琴 李春来 |
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| 作者单位： | 1. 莆田学院医学院,福建莆田351100 食品安全分析与检测教育部重点实验室福州大学,福建福州350108
2. 莆田学院医学院,福建莆田,351100 |
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| 基金项目： | 福建省教育厅科技计划项目（JA09197）; 食品安全分析与检测教育部重点实验室（福州大学）开放基金（FS10001）; 福建省科技计划重点项目（2011Y0048） |
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| 摘 要： | 采用Al 3＋增敏诺氟沙星的荧光强度,建立荧光分析法测定肉类食品中诺氟沙星的残留.优化了分析实验体系的最佳pH值、Al 3＋质量浓度和反应时间,利用荧光法测定,诺氟沙星的线性方程为F=15.89＋36.50C,线性范围在0.5～250ng.mL-1之间,方法检出限为0.032ng.mL-1,回收率在81.00%～99.31%之间.该方法简单、快速、灵敏、准确,可用于肉类食品中诺氟沙星含量的测定.
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| 关 键 词： | 诺氟沙星 荧光分析法 肉类 含量测定 |
Determination of Norfloxacin Residue in Meat Product by Fluorescence Spectrometry |
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| CAI Qihong,XU Guifen,RUAN Liqin,LI Chunlai.Determination of Norfloxacin Residue in Meat Product by Fluorescence Spectrometry[J].Journal of Shenyang University,2012,24(5):19-22. |
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| Authors: | CAI Qihong XU Guifen RUAN Liqin LI Chunlai |
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| Institution: | 1.Medical College,Putian University,Putian 351100,China;2.Key Laboratory of Analysis and Detection Technology for Food Safety,Ministry of Education,Fuzhou University,Fuzhou 350108,China) |
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| Abstract: | A spectrofluorimetric method for the determination of norfloxacin residue in meat product was described,based on Al3＋ can strengthen the relative fluorescence intensity of norfloxacin evidently.Under the optimum conditions of pH,Al3＋ concentration and reaction time,norfloxacin was detected by fluorescence spectrometry.The linear equation for norfloxacin was F=15.89＋36.50C,the linear range of the determination was 0.5 ng·mL-1 to 250 ng·mL-1,the detection limit was 0.032 ng·mL-1,and the recoveries were in the range of 81.00%~99.31%.This method is easy,swift,sensitive and accuracy.It can be applied to the determination of norfloxacin in meat product directly. |
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| Keywords: | norfloxacin fluorescence spectrometry meat product content determination |
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