| 离子束介导大豆DNA小麦低蛋白变异株系幼苗生长动态分析 |
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| 引用本文： | 路淑霞,姬生栋,岳春辉,兰烨. 离子束介导大豆DNA小麦低蛋白变异株系幼苗生长动态分析[J]. 河南师范大学学报(自然科学版), 2008, 36(6) |
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| 作者姓名： | 路淑霞 姬生栋 岳春辉 兰烨 |
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| 作者单位： | 河南师范大学生命科学学院,河南新乡453007 |
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| 基金项目： | 河南省自然科学基金(0511020600); 河南省植物学省级重点学科资助项目 |
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| 摘 要： | 经离子束介导大豆DNA后,获得了稳定遗传的第6代转基因小麦3个低蛋白变异株系,对3个低蛋白变异株系进行培养,分别在24 h,48 h,72 h和96 h观察和记录种子萌发和幼苗生长的情况,以未经离子束处理的相同小麦种子作为对照,研究发现:经离子束处理获得的3个低蛋白株系的种子萌发率与幼苗生长速度均明显高于对照,变异株系幼苗芽长、根长及侧根长与对照相比差异显著,t检验结果显示变异株系和对照相比芽长和跟长的差异程度均达到显著水平.
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| 关 键 词： | 离子束 大豆DNA 蛋白含量 小麦幼苗 萌发率 |
Seedling Growth Dynamic Analysis on Variant Strains of Low-Protein of Wheat Transferal with Soybean DNA by Ion Beam |
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| LU Shu-xia,JI Sheng-dong,YUE Chun-hui,LAN Ye. Seedling Growth Dynamic Analysis on Variant Strains of Low-Protein of Wheat Transferal with Soybean DNA by Ion Beam[J]. Journal of Henan Normal University(Natural Science), 2008, 36(6) |
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| Authors: | LU Shu-xia JI Sheng-dong YUE Chun-hui LAN Ye |
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| Affiliation: | LU Shu-xia,JI Sheng-dong,YUE Chun-hui,LAN Ye(College of Life Sciences,Henan Normal University,Xinxiang 453007,China) |
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| Abstract: | The transduction of soybean DNA by ion beam has produced three low protein content variant strains which have been stable genetic for 6 generations of wheat.Then they are observed and recorded separately on the condition of their germination and growth at 24 h,48 h,72 h and 96 h.The result shows that the three variant strains of low protein germination time are shorter than normal and the variations bear high germination rate and growth speed,and the seedling length and root and branch root length are remar... |
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| Keywords: | ion beam soybean DNA protein content wheat seedlings germination rate |
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