| Expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group |
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| 作者姓名： | BZHAO Wenran ZHONG Xuekuan ZHOU Lingwang |
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| 作者单位： | Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China,Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China,Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China |
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| 摘 要： | To express human phospholipid hydroperoxide glutathione peroxidase (PHGPx) in eukaryotic cells and to study its antiviral effect against Coxsackievirus B3m (CVB3m) in vitro， PHGPx cDNA was amplified from a human testis library using specific primers and cloned into expression vector pcDNA3.1His. Expression of PHGPx was performed in COS-1 cells. The antiviral effect was studied by the treatment of HeLa cells with the recombinant PHGPx. Results showed that the activity of PHGPx expressed in COS-1 cells was 5-fold higher than that in control group, and it inhibited the cytopathic effect on HeLa cells caused by CVB3m. It can be concluded that recombinant PHGPx expressed in COS-1 cells has antiviral effect against CVB3m in vitro.
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| 关 键 词： | PHGPx， gene expression， coxsackievirus group B， antiviral effect. |
Expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group |
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| BZHAO Wenran,ZHONG Xuekuan,ZHOU Lingwang.Expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group[J].Progress in Natural Science,2005,15(7):665-668. |
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| Authors: | BZHAO Wenran ZHONG Xuekuan ZHOU Lingwang |
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| Institution: | Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China,Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China,Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China |
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