| HPLC测定乌药叶中槲皮素的含量 |
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| 引用本文： | 王丽,吕圭源,王利.HPLC测定乌药叶中槲皮素的含量[J].北京联合大学学报(自然科学版),2011(2):50-52. |
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| 作者姓名： | 王丽 吕圭源 王利 |
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| 作者单位： | 1. 浙江中医药大学,杭州,310053
2. 浙江天台山乌药生物工程有限公司,浙江天台,317200 |
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| 摘 要： | 目的:建立高效液相色谱测定乌药叶中槲皮素含量的方法。方法:色谱柱(ZORBAX ECLIPSE XDB-C18,150 mm×4.6 mm,5μm),以甲醇∶0.4%磷酸=50∶50为流动相,流速:1.0 mL/min,检测波长:360 nm,柱温:30℃。结果:槲皮素在0.129μg～1.29μg内线性关系良好(r2>0.9999);重复性RSD为4.2%;平均加样回收率为101.0%,RSD为2.37%。结论:该方法专属性强,简便快捷,结果准确,可用作乌药叶槲皮素的含量测定。
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| 关 键 词： | 乌药叶 槲皮素 含量测定 高效液相 |
Determination of Quercetin in the Leaves of Lindera aggregate(Sims) Kosterm.by HPLC |
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| WANG Li,LV Gui-yuan,WANG Li.Determination of Quercetin in the Leaves of Lindera aggregate(Sims) Kosterm.by HPLC[J].Journal of Beijing Union University,2011(2):50-52. |
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| Authors: | WANG Li LV Gui-yuan WANG Li |
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| Institution: | WANG Li1,LV Gui-yuan1,WANG Li2(1.Zhejiang Chinese Medical University,Hangzhou 310053,China,2.Biological Engineering Co.,Ltd.,Zhejiang Tiantai Aggregata,Tiantai Zhejiang 317200,China) |
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| Abstract: | Objective To establish a method for the determination of quercetin in the leaves of Lindera aggregate(Sims) Kosterm.Methods Colum ZORBAX ECLIPSEXDB-C18,150 mm×4.6 mm,5 μm,the mobile phase consisted of methanol-0.4% phosphoric acid 50∶50 with a flow rate of 1.0 mL/min,the detection wavelength was monitored at 360 nm,and the column temperature was maintained at 30℃.Results The calibration curves of quercetin were linear in the range of 0.129 μg~1.29 μg.The reproducibity RSD was 4.18%,the average recovery of quercetin was 101.0%(RSD=2.37%).Conclsion The method is specific,simple,quick,accurate and can be used for the determination of quercetin in leaves of Lindera aggregata. |
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| Keywords: | leaves of Lindera aggregate(Sims) kosterm quercetin determination HPLC |
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