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| 一条在人睾丸中高表达的新的环指蛋白(RNF20) | |
| 引用本文： | 吴海,应康,顾少华,夏放,谢毅,杨泉胜,梅举,黄盛东.一条在人睾丸中高表达的新的环指蛋白(RNF20)[J].复旦学报(自然科学版),2000,39(6):663-666. |
| 作者姓名： | 吴海 应康 顾少华 夏放 谢毅 杨泉胜 梅举 黄盛东 |
| 作者单位： | 1. 复旦大学,生命科学学院,遗传学研究所,遗传工程国家重点实验室,上海,200433 2. 浙江大学,生物系,浙江,杭州,310027 3. 第二军医大学,第一附属医院,上海,200433 |
| 基金项目： | 上海市现代生物与新药产业发展基金资助项目!(98431912 1) |
| 摘 要： | 从人胎脑构建的cDNA文库中，通过大规模测序筛选的方法获得一条新基因，该基因蛋白序列含有1个环指（RING finger)基序及2个进核信号（nuclear localization signal NLS)序列，钭此基因命名为RNF20（RING Finger 20)，用放射杂交细胞系（Radial hybrid RH)方法定位此基因在人染色体的9q22上，Northern杂交表明，其mRNA在睾丸组织中高表达，用小鼠睾丸做原位杂交表明，小鼠的同源基因在精原细胞及初级精母细胞中高表达，未成年小鼠睾丸中的精原细胞没有发达，提示RNF20可能参与精子发生的调控作用。 |
| 关 键 词： | 环指蛋白 原位杂交 精子发生 人睾丸 |
RNF20,a Human Novel RING Finger Protein Expressed High in the Testis Located in 9q22.1-22.3 |
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| WU Hai,YING Kang,GU Shao-hua,XIA Fang,XIE Yi,YANG Quan-sheng,MEI Ju,HUANG Sheng-dong.RNF20,a Human Novel RING Finger Protein Expressed High in the Testis Located in 9q22.1-22.3[J].Journal of Fudan University(Natural Science),2000,39(6):663-666. | |
| Authors: | WU Hai YING Kang GU Shao-hua XIA Fang XIE Yi YANG Quan-sheng MEI Ju HUANG Sheng-dong |
| Institution: | WU Hai 1,YING Kang 1,GU Shao hua 1,XIA Fang 1,XIE Yi 1,YANG Quan sheng 2,MEI Ju 3,HUANG Sheng dong 3 |
| Abstract: | A novel RING finger gene was isolated from screening a human fetal brain cDNA library. In additional, two bipartite nuclear localization signals(NLS BP) were found in its protein sequence. The gene was located at chromosome 9q22 through RH (radiation hybird) mapping. The result of Northern blot using MTN (multi tissue Northern) indicated that it expressed in testis at very high level however, at very low lever in prostate and ovary. In situ hybridization of mouse testis indicated that the mouse homology of the RING finger gene was expressed high in spermatogonia and primary spermatocyte. These suggested that the RING finger gene was important in spermatogenesis. |
| Keywords: | RING finger protein(RNF) in situ hybridization(ISH) spermatogenesis |
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