| 哺乳动物胚胎冷冻技术研究进展 |
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| 引用本文： | 何万红,万五星,张国红. 哺乳动物胚胎冷冻技术研究进展[J]. 河北师范大学学报(自然科学版), 2002, 26(1): 85-90 |
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| 作者姓名： | 何万红 万五星 张国红 |
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| 作者单位： | 中学,河北石家庄050011河北师范大学生命科学学院,河北石家庄050016 |
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| 摘 要： | 报道了胚胎冷冻技术的发展及现状。根据它的发展分别介绍了慢冻慢解法、常规快速解冻法、玻璃化冷冻、1步细管法冷冻、1步冷冻和半胚冷冻,并且就胚胎冷冻保护剂、冷冻方法、解冷方法、冷冻保护剂的脱除及胚胎发育时期等对胚胎冷冻效果的影响进行了探讨。
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| 关 键 词： | 胚胎冷冻 超低温冷冻 慢冻慢解 快速冷冻 玻璃化冷冻 1步细管法 1步冷冻 半胚冷冻 |
| 文章编号： | 1000-5854(2002)01-0085-06 |
| 修稿时间： | 2001-09-23 |
Research Progress in Technology of Mammal Embryos Frozen |
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| HE Wan-hong ,,WAN Wu-xing ,ZHANG Guo-hong. Research Progress in Technology of Mammal Embryos Frozen[J]. Journal of Hebei Normal University, 2002, 26(1): 85-90 |
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| Authors: | HE Wan-hong WAN Wu-xing ZHANG Guo-hong |
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| Affiliation: | HE Wan-hong 1,2,WAN Wu-xing 2,ZHANG Guo-hong 3 |
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| Abstract: | To generalize progress and present situation in technology of embryo frozen.On the basis of development, there is showed slow freezing and slow ice-out,routine rapid freezing,virtification freezing,one-step canaliculus frozen,one-step freezing and semi-embryo frozen.The paper discusses the influence of embryo frozen-guard reagent of embryo frozen,frozen method,unfrozen method,get ride of protective reagent of embryo frozen and fetation period on frozen effect on embryo. |
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| Keywords: | embryo frozen cryopreservation slow frozen and slow ice-out rapid frozen virtification freezing one-step canaliculus frozen one-step frozen semi-embryo frozen |
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