AW915115参与大鼠再生肝星形细胞的Notch信号转导

为了解新基因AW915115在Notch信号通路中的作用及与大鼠肝再生的相关性,用Percoll密度梯度离心结合免疫磁珠分选方法分离大鼠再生肝的8种细胞,用Rat Genome 230 2.0芯片等检测上述细胞的Notch信号通路基因在大鼠肝再生中的表达变化,用BLAST、Microsoft Excel等软件分别分析新基因与已知基因的序列同源性和共表达关系,用生物信息学和系统生物学等方法分析上述基因参与的生理活动.结果表明,AW915115与活化Notch受体的N-乙酰葡糖基转移酶基因lfng同源,并且在2 h和12 h再生肝星形细胞中表达下调.根据上述基因的同源性和共表达关系推测,新基因AW915115参与大鼠再生肝星形细胞的Notch信号转导.

AW915115 is Involved in Notch Signaling Cascade of Hepatic Stellate Cell during Rat Liver Regeneration

To explore the role of new gene AW915115 in Notch signaling cascade and the relevance between AW915115 and rat liver regeneration,in this investigation,Percoll density gradient centrifugation combined with immunomagnetic beads method were employed to sorted out 8 kinds of rat regenerating liver cells.The high throughput methods including Rat Genome 230 2.0 array were applied to detect expression changes of Notch signaling cascade genes in the cells,and the new genes and their known homology and co-expression relation and the possible physiological activities were analyzed by BLAST,Microsoft Excel programs and the methods of bioinformation and systematic biology et al.It was found that AW915115 and N-acetylglucosyl-transferase gene lfng that activates Notch recepter were homologous and down-regulated in stellate cell at 2h and 12h after partial hepatectomy.Based on the homology and coexpression of above genes,the new gene AW915115 was involved in Notch signaling cascade of hepatic stellate cell during rat liver regeneration.