MTT法测定人表皮癌细胞的抗药性

在用ＭＴＴ测定细胞存活性率以研究人表皮细胞ＫＢ－３－１及其抗阿霉素细胞株ＫＢ－Ａ－１对化疗药物阿霉素，长春花碱和秋水仙碱的多药抗性时，发现培养基中血清浓度的变化对ＭＴＴ法测定结果有影响，吸光值在血清浓度为ｗ＝０．０２时最高，ｗ＝０．３０时最低，ｗ＝０．０５－０．２０之间波动不大。而不加血清使甲 溶解困难而产生误差。培养基中的血清（常为ｗ＝０．１０）不影响ＭＴＴ法的结果，在完全培养基中用ＭＴＴ法测定

Determination of Multidrug Resistance of Human KB Carcinoma Cells by MTT Colorimetric Assay

To determine the relative resistances of KB A 1 cells to the parent cell line KB 3 1,MTT colorimetric assay was used to evaluate the cell growth and survival.In the experiment,It was found that the serum can affect the result of MTT assay,and the optical density reached the maximum with the serum concentration of w =0.02,and the minimum with w =0.30.While at the concentrations between w =0.05~0.20 it fluctuated a little.When no serum was added,the formazan product could not be easily dissolved and the error was inevitably introduced.So,in the ordinary range around w =0.10,the addition of serum to the reaction medium will not cause error to the MTT assay and the experiment can be done easily.This MTT assay was used to perform drug sensitivity assays of KB 3 1 and its drug resistant mutant,KB A 1.The relative resistances of KB A 1 to colchicine,vinblastine and adriamycin are 137,45 and 167 respectively compared with KB 3 1.