| 中华芦荟成熟叶组织培养的研究 |
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| 引用本文： | 姚发兴,陈志芬,江威,何随箔,白欣,周宇江,谢茜,董昌金.中华芦荟成熟叶组织培养的研究[J].湖北师范学院学报(自然科学版),2008,28(4). |
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| 作者姓名： | 姚发兴 陈志芬 江威 何随箔 白欣 周宇江 谢茜 董昌金 |
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| 作者单位： | 湖北师范学院,生命科学学院,湖北,黄石,435002 |
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| 基金项目： | 湖北省教育厅重大项目
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| 摘 要： | 以中华芦荟成熟叶为外植体,接种于9组不同2,4-二氯苯氧乙酸(2,4-D)和6-苄基腺嘌呤(6-BA)浓度配比的脱分化培养基中,诱导外植体的脱分化形成愈伤组织。研究表明,中华芦荟叶愈伤组织的最佳培养基为MS+0.5mg/L(2,4-D)+3mg/L(6-BA)+30g/L蔗糖+10g/L琼脂+1.0g/L活性炭;诱导不定芽的适宜培养基为MS+1mg/L(2,4-D)+3mg/L(6-BA)+30g/L蔗糖+10g/L琼脂+0.7g/L活性炭;及诱导生根的适宜培养基为1/2MS+0.2mg/Lα-萘乙酸(NAA)+20g/L蔗糖+10g/L琼脂+0.5g/L活性炭,生根率达100%.
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| 关 键 词： | 中华芦荟 愈伤组织 正交实验 最佳培养基 |
Study for tissue culture of leaf from Aloe vera L. var. chinesis(Haw.)Berger |
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| YAO Fa-xing,CHEN Zhi-fen,JIANG Wei,HE Sui-bo,BAI Xin,ZHOU Yu-jiang,XIE Qian,DONG Chang-jin.Study for tissue culture of leaf from Aloe vera L. var. chinesis(Haw.)Berger[J].Journal of Hubei Normal University(Natural Science),2008,28(4). |
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| Authors: | YAO Fa-xing CHEN Zhi-fen JIANG Wei HE Sui-bo BAI Xin ZHOU Yu-jiang XIE Qian DONG Chang-jin |
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| Institution: | YAO Fa-xing; CHEN Zhi-fen; JIANG Wei; HE Sui-bo; BAI Xin; ZHOU Yu-jiang; XIE Qian; DONG Chang-jin(College of Life Science; Hubei Normal University; Huangshi 435002; China); |
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| Abstract: | In our research,explants from mature leaves of Aloe vera L.var.chinese(Haw.) Berger were cultured in 9 kinds of mediums containing different 2,4-D and 6-BA to induce the form of callus from undifferentiated tissues.The result indicated that the optimum proportion of the cultured medium for the callus,tufted-buds,roots of Aloe vera L.var.chinese(Haw.) was MS+0.5mg/L(2,4-D)+3mg/L(6-BA)+30g/L sucrose+10g/L agar+ 1.0g/L(AC),MS+1mg/L(2,4-D)+3mg/L(6-BA)+30g/L sucrose+10g/L agar+0.7g/L(AC),1/2MS+0.2mg/L(NAA)+20g/L... |
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| Keywords: | Aloe vera L var chinese（Haw ）Berger callus orthogonal test optimum cultured medium |
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