动态微波辅助萃取与高效液相色谱联用测定金银花中的绿原酸

通过流动注射的方法将动态微波辅助萃取与高效液相色谱相连接, 用于测定金银花中的绿原酸. 萃取过程在一个循环体系中完成， 萃取后, 通过采样环采集到的20 μL样品被流动相载带到色谱系统进行分离检测. 在最佳实验条件下得到的检出限和测定下限分别为0.25和0.83 μg/mL, 日内和日间精密度(RSD)分别为3.4%和4.1%, 平均加标回收率为98.1%.

Determination of Chlorogenic Acid in Flos Lonicerae Japonicae via Dynamic Microwave-assisted Extraction Coupled On-line with High-performance Liquid Chromatography

A fast method based on dynamic microwave-assisted extraction (DMAE) coupled on-line with high performance liquid chromatography (HPLC) by means of a flow injection interface has been developed for the determination of chlorogenic acid in Flos Lonicerae japonicae. The extraction was performed in a recirculating system. When a number of extraction cycles were completed, the fractional extract (20 μL) was driven to the analytical column by mobile phase and was measured by a UV detector. The extraction conditions were examinedand optimized. The detection and quantification limits of chlorogenic acid obtained at optimized conditionsare 0.25 and 0.83 μg/mL, respectively. The intra day and inter day precisions (RSD) obtainedare 34% and 41%, respectively. The mean recovery of chlorogenic acid is 98.1%. Compared with ultrasonic extraction used in the Chinese pharmacopoeia, the proposed method reduces the amount of solvent, provides more rapid measurement and high extraction yield.