| 反胶束中脂肪酶CSL催化性能的研究 |
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| 引用本文： | 张微,阎云.反胶束中脂肪酶CSL催化性能的研究[J].东北师大学报(自然科学版),1999(4):33-37. |
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| 作者姓名： | 张微 阎云 |
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| 作者单位： | 东北师范大学化学学院!吉林长春，130024，东北师范大学化学学院!吉林长春，130024，东北师范大学化学学院!吉林长春，130024，东北师范大学化学学院!吉林长春，130024，东北师范大学化学学院!吉林长春，130024 |
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| 基金项目： | 国家自然科学基金!2973009 |
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| 摘 要： | 以葵花籽油为底物，考察了未见报道的国产脂肪酶ＣＳＬ在ＡＯＴ／异辛烷反胶束体系中的催化性能，当底物体积分数为１０％，ＰＨ＝８．０，Ｔ＝３１３Ｋ，水与表活性剂的浓度比Ｗ０＝１２时，脂肪酶的催化活力最高；而且，当底物体积分数为２０％时，仍未发生抑制现象。
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| 关 键 词： | 反胶束 脂肪酶CSL 催化性能 水解反应 |
Research on the catalysis characteristics of CSL in reversed micelle system |
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| ZHANG Wei, YAN Yun, LIU Jing-lin,CHU Ying, WU Zi-sheng.Research on the catalysis characteristics of CSL in reversed micelle system[J].Journal of Northeast Normal University (Natural Science Edition),1999(4):33-37. |
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| Authors: | ZHANG Wei YAN Yun LIU Jing-lin CHU Ying WU Zi-sheng |
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| Abstract: | The catalysis characteristics of candida Sp lipase (CSL), which was made in China, had been researched when the sunflower oil was used as substrate in the AOT/isooctane system. Under the following conditions CSL had the highest activity: the concentration of the substrate was 10%, pH value was 8.0, the temperature is 313 K, W0 value was 12. It was confirmed that the catalysis characteristics of CSL in reversed micelle was better when compared with CSL in oil/water diphase system. |
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| Keywords: | reversed micelle candida Sp lipase (CSL) catalysis characteristics |
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