| 发酵法产酶生产几丁寡糖的工艺研究 |
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| 引用本文： | 乔兴忠,李永娴,汤熙翔.发酵法产酶生产几丁寡糖的工艺研究[J].厦门大学学报(自然科学版),2010,49(2). |
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| 作者姓名： | 乔兴忠 李永娴 汤熙翔 |
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| 作者单位： | 国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,福建,厦门,361005 |
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| 基金项目： | 人事部回国留学人员基金，福建省科技计划重点项目 |
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| 摘 要： | 利用前期获得并经基因工程改造的一株高效几丁质酶降解菌进行发酵产酶法降解几丁质胶体与膜分离相耦合制备几丁寡糖的中试研究.经过中试发酵工艺、酶反应工艺、分离纯化工艺的研究,最终确定了一条高效制备几丁寡糖的中试规模工艺流程,该流程中反应分离一体化,过程实现连续操作,降解酶可以连续使用,提高了收率,降低了成本,有利于实现产业化.通过该流程制备的几丁寡糖混合物含几丁2、3、4糖分别为71.3%、23.5%、2.4%.
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| 关 键 词： | 发酵 酶法 几丁寡糖 |
Chitin Oligosaccharides Produced by Enzyme Method |
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| QIAO Xing-zhong,LI Yong-xian,TANG Xi-xiang.Chitin Oligosaccharides Produced by Enzyme Method[J].Journal of Xiamen University(Natural Science),2010,49(2). |
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| Authors: | QIAO Xing-zhong LI Yong-xian TANG Xi-xiang |
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| Institution: | QIAO Xing-zhong,LI Yong-xian,TANG Xi-xiang(Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography State Oceanic Administration,Xiamen 361005,China) |
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| Abstract: | Chitin oligosaccharides was produced by chitinases digestion of chitin colloid coupling with membrane separation.The chitinases was produced by fermentation through wild(aeromonas caviae CB101) bacteria changed by our group.A method suitable for industrialization was suggested through detail fermentation,enzyme digestion,and separation study.The character of this method is digestion and separation can be operated at same time in series.The purity of N-Acetylchitooligosaccharides(dimer,trimer,tetramer) produ... |
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| Keywords: | fermentation enzyme method chitin oligosaccharides |
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