| 抗癌酶制剂L—天冬酰胺酶在甲壳素上的固定化 |
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| 引用本文： | 周纪宁,李永丰.抗癌酶制剂L—天冬酰胺酶在甲壳素上的固定化[J].华东理工大学学报(自然科学版),1999,25(5):438-441,445. |
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| 作者姓名： | 周纪宁 李永丰 |
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| 作者单位： | [1]华东理工大学化工学院 [2]华东理工大学应用生化系 |
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| 摘 要： | 研究了以甲壳素为载体固定化Ｌ－天冬酰胺酶的最适条件，探讨了固定化Ｌ－天冬酰胺酶的一系列理化性质。交联剂用量、缓冲液ｐＨ值和载体活化时间均对Ｌ－天冬酰胺酶的固定化有一定影响，得到的固定化Ｌ－天冬酰胺酶的活力回收可达２５．４％。固定化Ｌ－天冬酰胺酶理化性质的实验研究表明，酶经固定化后，对蛋白水解酶的稳定性及存贮稳定性均较游离酶有很大程度的提高。
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| 关 键 词： | L－天冬酰胺酶 甲壳素 固定化酶 抗癌酶制剂 |
Immobilization of Antitumor Agent L asparaginase on Chitin |
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| ZHOU Ji ning ,JIN Hao ,JIANG Ti qian ,LI yong feng.Immobilization of Antitumor Agent L asparaginase on Chitin[J].Journal of East China University of Science and Technology,1999,25(5):438-441,445. |
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| Authors: | ZHOU Ji ning JIN Hao JIANG Ti qian LI yong feng |
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| Institution: | ZHOU Ji ning 1,JIN Hao 1,JIANG Ti qian *1,LI yong feng 2 |
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| Abstract: | The optimum immobilization conditions and some properties of immobilized L asparaginase with chitin as the support were studied. The dosage of crosslinking agent, buffer pH value and activation time of carrier played some importat roles in L asparaginase immobilization. The maximum relative activity of immobilized L asparaginase could reach 25.4%. The researches on properties of immobilized L asparaginase demonstrated that the resistance to pepsin hydrolysis and storage stability of immobilized L asparaginase were increased greatly compared with that of free enzyme. |
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| Keywords: | L asparaginase chitin glutaric dialdehyde immobilized enzyme |
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