| NaGdF_4:Yb;Er纳米颗粒的合成及对HeLa细胞的生物标记 |
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| 引用本文： | 密丛丛,高环宇,何喆,徐淑坤.NaGdF_4:Yb;Er纳米颗粒的合成及对HeLa细胞的生物标记[J].东北大学学报(自然科学版),2011,32(7):981-984. |
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| 作者姓名： | 密丛丛 高环宇 何喆 徐淑坤 |
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| 作者单位： | 东北大学理学院,辽宁沈阳,110819 |
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| 基金项目： | 国家自然科学基金资助项目(20875011); 中央高校基本科研业务费专项资金资助项目(N090605002) |
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| 摘 要： | 以稀土硬脂酸盐为前驱体,采用溶剂热法合成了单分散的NaGdF4:Yb,Er上转换荧光纳米颗粒.通过透射电子显微镜(TEM)、X射线衍射仪(XRD)、荧光(FL)光谱仪、磁滞回线(MHL)等对合成的样品进行了表征.所合成的纳米颗粒粒径均一,平均粒径为20 nm左右,形状规则,结晶度高,发光性能良好,同时具有优越的顺磁性.利用经典的Stober法对纳米颗粒进行了表面氨基修饰,将修饰后的纳米颗粒与转铁蛋白结合制成探针,实现了对HeLa细胞的标记与成像.标记结果表明:该方法特异性较好,且红外光激发可有效避免来自生物样品的自体荧光干扰.
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| 关 键 词： | 上转换 荧光 磁性 溶剂热法 NaGdF4 |
Synthesis of NaGdF_4:Yb,Er Nanoparticles and Their Application in Cell Biolabeling |
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| MI Cong-cong,GAO Huan-yu,HE Zhe,XU Shu-kun.Synthesis of NaGdF_4:Yb,Er Nanoparticles and Their Application in Cell Biolabeling[J].Journal of Northeastern University(Natural Science),2011,32(7):981-984. |
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| Authors: | MI Cong-cong GAO Huan-yu HE Zhe XU Shu-kun |
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| Institution: | MI Cong-cong,GAO Huan-yu,HE Zhe,XU Shu-kun(School of Sciences,Northeastern University,Shenyang 110819,China.) |
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| Abstract: | The monodispersed NaGdF4:Yb,Er nanoparticles were synthesized via a solvothermal approach.Rare-earth stearate was used as the precursor.The properties of as-prepared nanoparticles were characterized by transmission electron microscopy (TEM),X-ray diffraction(XRD),fluorescence spectra and magnetic hysteresis loop(MHL).Experimental results showed that the nanoparticles are single crystal hexagonal phases with an average size of around 20 nm,exhibiting good superparamagnetic and excellent up-conversion fluores... |
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| Keywords: | up-conversion fluorescence magenetic solvothermal NaGdF4 |
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