Lats基因敲除小鼠胚胎玻璃化冷冻的研究

用冷冻麦管、细胞冷冻管分别采用一步法和二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻 ,胚胎解冻后 ,囊胚发育率分别为 90 %、90 1%、89 2 %和 91 4 % ;移植后着床率分别为 :5 0 0 %、4 7 1%、4 3 4 %和 4 6 5 % ,差异不显著 ;并且与对照组相比差异也不显著。因此 ,玻璃化冷冻可以作为Lats鼠保种方法之一

Study on Vitrified Method of Lats Knock-out Mice Embryo

Adopting one step and two|step method,we use Lats knock|out mice embryo to optimize the condition of vitrified with straw and cell freezing tube.The developing percentage of thawed embryo reached 90%,90.1%,89.2% and 91.4%,respectively.The implantation ratio reached 50%,47.1%,43.4% and 46.4%,respectively.The difference is not remarkable compared to the control and each other.So the Lats knock|out mice can be preserved by vitrification.