| 喜树内生真菌的分离及产10-羟基喜树碱菌的鉴定 |
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| 引用本文： | 闵长莉,袁萍. 喜树内生真菌的分离及产10-羟基喜树碱菌的鉴定[J]. 华东师范大学学报(自然科学版), 2009, 2009(5): 94-99 |
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| 作者姓名： | 闵长莉 袁萍 |
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| 作者单位： | 皖西学院化学与生命科学系,安徽六安,237012;上海师范大学生命与环境科学学院,上海,200234 |
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| 摘 要： | 从喜树（Camptotheca acuminate Decne）树皮中分离、纯化得到6株内生真菌,经摇瓶培养后,采用TLC法与HPLC法对其菌丝体提取物进行分析,结果表明其中1株内生真菌(XK002)产10-羟基喜树碱（10-hydroxycamptothecin,HCPT）,将此菌株命名为XK002,其10-羟基喜树碱产量为410 μg/L.对菌株XK002菌落、菌丝体和孢子的有无进行了研究,表明菌株XK002属于无孢菌群(Mycelia sterlia).
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| 关 键 词： | 喜树内生真菌 分离 鉴定 10-羟基喜树碱 |
| 收稿时间： | 2008-12-19 |
| 修稿时间： | 2009-03-04 |
Isolation and identification of a 10-hydroxycamptothecin-producingendophytic fungus from Camptotheca acuminate Decne (Chinese) |
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| MIN Chang-li,YUAN Ping. Isolation and identification of a 10-hydroxycamptothecin-producingendophytic fungus from Camptotheca acuminate Decne (Chinese)[J]. Journal of East China Normal University(Natural Science), 2009, 2009(5): 94-99 |
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| Authors: | MIN Chang-li YUAN Ping |
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| Affiliation: | 1. Department of Chemistry and Life Science, West Anhui University, Liuan Anhui 237012, China; 2. Department of Biology and Environment Science, Shanghai Normal University,Shanghai 200234, China |
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| Abstract: | Six endophytic fungi isolated from Camptotheca acuminate Decne were cultured in taper flask under shaking. The organic extracts of mycelium were analyzed with thin layer chroma-tography (TLC) and high performance liquid chromatography (HPLC). The results showed one of the strains produced the 10-hydroxycamptothecin (HCPT) with output of 410 f*g/L and was named XK002. Morphological observation indicates that XK002 belongs to Mycelia sterlia. |
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| Keywords: | endophytic fungi of Camptotheca acuminate Decne isolation identification 10-hydroxycamptothecin |
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