| A novel multi-functional cell-based microphysiometer |
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| 作者姓名： | XU Ying XU Gaixi LIU Qingjun CAI Hu LI Yan LI Rong WANG Ping |
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| 作者单位： | 1. Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; 2. State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China |
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| 基金项目： | 国家自然科学基金;国家重点实验室基金;浙江省教育厅资助项目 |
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| 摘 要： | This paper presents a novel multi-functional microphysiometer for simultaneous measurements of several extracellular ion concentrations and action potential measurement in living cells based on MLAPS (multi-light addressable potentiometric sensor). In the microphysiometer, sorts of sensitive membranes are illuminated in parallel with n light sources at working frequencies, and the response amplitudes of each frequency component can be measured on-line by parallel processing algorithm. In the experiments, the relations of the extracellular environmental H+, Na+, K+, Ca2+ under the effects of western medicines (dilantin, phenobarbital sodium) and Chinese drugs (scutellaria, medlar, hemlock parsley) were analyzed, and the effects of several drugs were evaluated. Moreover, the action potential signals of different cell types (cardiac myocytes and neurons) could be measured and analyzed by LAPS. By detecting these parameters, the system can monitor the real-time process of the cell metabolism and action potential, observe the functional responses of different kinds of membrane-bound receptors, and evaluate the activities of drugs.
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| 关 键 词： | multi-functional microphysiometer cell-based biosensor cellular ion concentration measurement cellular action potential. |
A novel multi-functional cell-based microphysiometer |
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| XU Ying,XU Gaixi,LIU Qingjun,CAI Hu,LI Yan,LI Rong,WANG Ping.A novel multi-functional cell-based microphysiometer[J].Progress in Natural Science,2006,16(8):808-816. |
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| Authors: | XU Ying XU Gaixia LIU Qingjun CAI Hua LI Yan LI Rong WANG Ping |
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| Institution: | 1. Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China;State Key Laboratory of Transducer Technology, Chinese Academy of Sciences,Shanghai 200050,China
2. Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | This paper presents a novel multi-functional microphysiometer for simultaneous measurements of several extracellular ion concentrations and action potential measurement in living cells based on MLAPS (multi-light addressable potentiometric sensor). In the microphysiometer, sorts of sensitive membranes are illuminated in parallel with n light sources at working frequencies, and the response amplitudes of each frequency component can be measured on-line by parallel processing algorithm. In the experiments, the relations of the extracellular environmental H+, Na+, K+, Ca2+ under the effects of western medicines (dilantin, phenobarbital sodium) and Chinese drugs (scutellaria, medlar, hemlock parsley) were analyzed, and the effects of several drugs were evaluated. Moreover, the action potential signals of different cell types (cardiac myocytes and neurons) could be measured and analyzed by LAPS. By detecting these parameters, the system can monitor the real-time process of the cell metabolism and action potential, observe the functional responses of different kinds of membrane-bound receptors, and evaluate the activities of drugs. |
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| Keywords: | multi-functional microphysiometer cell-based biosensor cellular ion concentration measurement cellular action potential |
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