| 水稻悬浮系细胞的建立及植株再生 |
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| 引用本文： | 王俊丽,宋陆铧,薛静,廖祥儒,杜建芳,陈丕铃.水稻悬浮系细胞的建立及植株再生[J].河北大学学报(自然科学版),1999(4). |
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| 作者姓名： | 王俊丽 宋陆铧 薛静 廖祥儒 杜建芳 陈丕铃 |
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| 作者单位： | 河北大学生物工程研究所!河北保定，071002，河北大学生物工程研究所!河北保定，071002，河北大学生物工程研究所!河北保定，071002，河北大学生物工程研究所!河北保定，071002，河北大学生物工程研究所!河北保定，071002，河北大学生物工程研究所!河北保定，071002 |
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| 摘 要： | 水稻（辽盐9）种子在LS＋2，4－D（2，4-二氯苯氧乙酸）2．0～3．0mg/L的培养基上进行诱导培养，经3～4次继代后，即可得到淡黄色颗粒性愈伤组织；将该种愈伤组织在LS＋2，4－D2．0～3．0mg／L＋LH（水解乳蛋白）300mg/L的液体培养基上进行振荡培养，经4～5次继代后，即可建立起良好的细胞悬浮系；培养基中附加2．0～2．5mg／LKT，使愈伤组织和悬浮细胞均可再生，其再生率分别为15．9％～17．9％和18．1％～20．8％。
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| 关 键 词： | 水稻 愈伤组织 悬浮细胞 植株再生 |
Establishment of Suspension Cells and Plant Regeneration of Rice |
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| WANG Jun-li,SONG Lu-hua,XUE Jing,LIAO Xiang-ru,DU Jian-fang,CHEN Pi-ling.Establishment of Suspension Cells and Plant Regeneration of Rice[J].Journal of Hebei University (Natural Science Edition),1999(4). |
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| Authors: | WANG Jun-li SONG Lu-hua XUE Jing LIAO Xiang-ru DU Jian-fang CHEN Pi-ling |
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| Abstract: | The rice seeds(Liao Yan 9)were cultured and induced in the LS medium.The results showed that the medium of LS+2,4-D2.0～3.0mg/L could induce growth of callus. After 3～4times subculture,the callus induced was light yellow, grainy and available for suspension culture. The calli were inoculated into the medium of LS+2,4-D2.0～3.0 mg/L+LH300 mg/L and cultured on the rotatory shaker. After 4～5 times subculture, the suspension cells could be obtained. KT was suitable for plants regeneration of rice, and the concentration of it was about 2.0～2.5mg/L. The regeneration frequence of calli and suspension cells was 15.9%～17.9% and 18. 1%～20. 8% respectly. |
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| Keywords: | rice callus suspension cell plant regeneration |
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