碱性磷酸酶在体外钙化中的作用

报道了用碱性磷酸酶抑制剂左旋咪唑和缓钙化剂偕二磷酸钠，通过体外钙化实验，跟踪测试ＡＫＰ比活力及＾４５Ｃａ在佝偻病从鼠的骺骨生长板中的掺入。结果表明，钙化与酶失活，缺乏Ｃａ＾２＋或Ｐｉ的条件下不会发生，ＡＰＫ活力对钙化的发生是必需的，ＡＫＰ尖力超高，钙化速度越快，同时，钙化会加速ＡＫＰ失活，钙化速度越快，ＡＫＰ活力降低越快。

THE ROLE OF ALKALINE PHOSPHATASE IN THE PROCESS OF CALCITICATION IN VITRO

Growth plate cortilage of rachitic rat was used to studied factors influencing biological calcification.Levamisole,a inhibitor of alkaline phosphatase (AKP) and disodium ethanehydroxydiphosphonate (Na 2EHDP),a anticalcification agent,were used to study the velation between AKP and calafication.Measurements of specific activity of AKP and 45 Ca uptuke by rachitic cartilage slices from metastable calcium phosphate solution indicated that heat and without Ca 2 or Pi in the solution inhibited calcification almost totally,and levamisole and Na 2EHDP inhibited calcification partily.The results suggest that AKP is essential for biologic calcification.In the process of biologic calcification,The acticity of AKP will be loss gradually,The faster the speed of calcification is,the faster the loss of AKP activity is.